Obtaining An Anisotropy Image - Zeiss LSM 880 Operating Manual

ZEISS Center Screen Area / Image Containers - Display and Image Analysis
6.20.3

Obtaining an Anisotropy Image

Set up a multitrack configuration with alternating analyzers: P in track 1, S in track 2, leaving all other
settings equal (see Fig. 749). Assign track 1 to Ch1-T1, track 2 to Ch1-T2. Alternatively assign to Ch2-T1
and Ch2-T2 or ChS1-T1 and ChS1-T2, whatever physical channel you want to use.
Fig. 749 Channels tool with Ratio Type 1
formula selected for the Ratio1
channel using a G factor of 1.18
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CHAPTER 1 - SYSTEM OPERATION
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Fig. 750 Image calculator with anisotropy
formula and a chosen G-factor of
1.18 with
selected
LSM 880
absolute intensities
10/2014 V_01