Selection Of Laser Lines - Zeiss LSM 880 Operating Manual

ZEISS
Fig. 265 Light Path tool with laser submenu
Fig. 266 Visible Light Laser panel
Fig. 267 Invisible Light Laser panel
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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5.3.2.4

Selection of Laser Lines

• Press the Laser button to display the laser
control expansion
Alternatively you have access to the Laser Lines
in the Acquisition tab. They provide settings
for:
− Lasers for laser power used in measurement
and pinhole adjustment,
− Bleach for laser power used in bleaching
Since the expansion submenu will close when
clicking in other areas, we recommend to use the
Laser Lines from the Acquisition tab, if you want
to adjust the count rate.
Lasers
• Press the Visible Light or Invisible Light tab to
display the laser
measurements and pinhole adjustments (Lasers
area) or bleaching (Bleach area) (Fig. 266). Press
again to close the panel.
• Activate the lasers you want to use by checking
the corresponding boxes. The lasers with sliders
will appear. Set the requested laser attenuation
(%) using the sliders, the arrows, or typing in
the display field.
Note, that lasers for measurements and
(pre-)bleach can be selected independently
from each other.
If in AOTF Dampening a factor different
from 100 % was chosen, the set AOTF value
in Lasers and Bleach will be reduced by that
percentage.
Note, that lasers for measurements and pre-
bleach can be selected independently from
each other.
The same options are available for the
405 and 440 lasers and the NLO laser, if
available, in the Invisible Light laser
selection box (Fig. 267).
LSM 880
submenu (Fig. 265).
control panel used
10/2014 V_01
for