Zeiss LSM 880 Operating Manual page 5

LSM 880
IMPORTANT NOTES FOR CHAPTER 1
• Visual Macro Editor does not allow saving .czi file format.
• Auto Z correction function works with laser powers smaller than 0.5 % but does not display the
changes even if Enable Test is active.
• Do not use "Double click in image" function for shuttle and find as this does move stage when
keeping the mouse button pressed.
• Processed PALM data do not show up as image in the Shuttle and Find View tab, use unprocessed
data instead.
• Do not use "Double click in image" function for shuttle and find as this does move stage even without
calibration data.
• Creating a new block moves the stage in Z to a lower position. Duplicate an existing block instead to
not lose the focus.
• Do not reuse images which have been acquired with polarization filters use for acquisition. The filters
cannot be removed from the beam path afterwards and ZEN has to be restarted if that is needed.
• The Z coordinate in the info view refers to the center position for Position Experiments or the first
image of the stack for Non-Position Experiments.
• Using Fast Z Line mode in combination with a Multitrack does result in wrong results. Do not check
fast Z line or avoid multi-tracking.
• Multi-positions will not be concatenated; the positions dimension is lost.
• Tuning a multiphoton laser with inbuilt dispersion compensation to a different wavelength for
bleaching might result in the first image(s) following the bleach event not being final as the GDD can
still be moving. This can be set via database. Contact your ZEISS representative on details if that
function is of interest for you.
• Using arrow, line or scale bar as graphics tool in non-processed Airyscan data (Airyscan View) does
change table parameters in an unspecified way. Use these tools only for processed data.
• Concatenating SR mode and VP mode acquired datasets using modify series or the concatenate macro
produces faulty results.
• Spot select for spot imaging or bleaching should only be used within one image container. Applying it
to images in different containers may result in malposition of the spots.
• Using the split mode of the 3D view does not work in combination with measurements in 3D. Use
either or for display or measurement.
• Do not use ILEX mode other than when Airyscan detector is set active.
• When using LSM and SIM imaging in multitrack mode, use the maximal pixel resolution for the
camera to be able to apply a fit function to the SIM image.
• Particle tracking requires non processed PALM data as input. Processed data produce no results.
• For wide-field imaging, set the halogen lamp for illumination during continuous scan to adjust before
starting the final scan. Settings of the lamp without scanning might not be effective for the image
acquisition.
• When using LSM and SIM imaging in multitrack mode, the processing of the SIM image will then lead
to a loss of the last image of the LSM acquisition. Split the images before processing the SIM image(s).
10/2014 V_01
IMPORTANT NOTES
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ZEISS
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