Setting Individual Offset Values For Positions In Position List Mode; Lowering Objective When Moving The Stage - Zeiss LSM 880 Operating Manual

ZEISS
5.2.20.3

Setting Individual Offset Values for Positions in Position List Mode

The Focus Devices and Strategy tool contains the controls for maintaining focus stability when using
the Positions tool (e.g. selection of autofocus mode and activity intervals).
If Show all is active for the Positions tool, it is possible to assign individual offset values to each position
when using the Position List mode. This feature is exclusively available if the autofocus Reflection mode
is selected in Focus Devices and Strategy.
To modify the offset value of individual positions a position is selected by mouse click.
Next, the offset value is either typed into the offset spin box or determined automatically after pressing
the
Offset values of individual positions must be modified within the context of the Positions tool and not in
Focus Devices and Strategy.
If individual offset values are specified in position lists, these values are ignored upon switching to an
autofocus mode other than Reflection.
In the case of the method Sample Carrier, ZEN assumes identical offset values across all positions in
arrayed samples. Use the tool Focus Devices and Strategy to specify the offset value when using this
method. See tool description of Focus Devices and Strategy for details on autofocus modes and offset
values (section Acquisition Parameter – Focus Devices and Strategy).
Editing offset values of individual positions is available in Position List for the Reflection autofocus mode
5.2.20.4

Lowering Objective when Moving the Stage

Some acquisition experiments require the objective lens to be lowered (or raised depending on the
microscope) before moving the scanning stage to the next position (e.g. required if certain plate formats
are used).
With the tool Positions, this is possible when activating
This feature is available to Position List and Sample Carrier if Show all is active.
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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10/2014 V_01