Using Gaasp Detectors And The Temperature Controlled Side Pmt For Photon Counting Imaging - Zeiss LSM 880 Operating Manual

LSM 880
5.2.9.3 Using GaAsP Detectors and the
temperature controlled side PMT for
Photon Counting Imaging
In the LSM Imaging Setup tool activate any GaAsP
detector like BiG.2, the 32 channel GaAsP Array
detector or the temperature controlled side PMT
(Ch2).
Select Photon Counting for the detector in the
Channels Tool (Fig. 159).
In photon counting imaging a Digital Offset and
Digital Gain slider is available, which can be used to
adjust brightness and contrast of the image during
scanning.
In photon counting mode an artificial maximum of
detectable photons is set. For all GaAsP detectors and
the side PMT (CH2) this value is defined by
CR /digital gain (CR
max max
by the software, which is 4 MHz for the BiG.2 and
Ch2 and 2 MHz for CHs (32 channel GaAsP Array).
Higher count rates are regarded as saturation. Note
that too much light on the detectors (higher count
rates for some ms) leads to detector shut down to
avoid damage.
(1) Display of Photon Count Images
There are two ways photon counting images can
be displayed in the LSM software, in analog (grey
value) or counting (raw data) mode.
You can toggle between the two modes in the
Maintain area Options / System Options in the
Hardware tab by deactivating or activating the
Keep raw data for photon count detectors
check box (Fig. 160).
If the box is checked, you will obtain the photon
counts, if unchecked, grey values corresponding to
photon counts.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
= maximal count rate defined
000000-2071-464
Fig. 159 Channels tool – Photon counting
mode for GaAsP detector
Fig. 160 Hardware tab in the Systems
Options tool/Maintain tab
ZEISS
121