Overlap Coefficient, Overlap Coefficient After Manders; Profile View - Zeiss LSM 880 Operating Manual

ZEISS Center Screen Area / Image Containers - Display and Image Analysis
6.11.4

Overlap Coefficient, Overlap Coefficient after Manders

(Manders, Verbeek and Aten, J. Microscopy 169:375-382, 1993)
∑
Ch 1 * Ch
i
=
i
r
∑ ∑
2
( Ch 1 ) *
i
i i
Another parameter used to quantify colocalization in image pairs
−
Insensitive to differences in signal intensities between the two channels, photo-bleaching or
−
amplifier settings
Value range 0 – 1 (0: no colocalization, 1:all pixels colocalize)
Fig. 671 View Option control block - Profile
View
control block is shown in Fig. 671.
Any changes done with this View Option control block are effective immediately.
The content of the overlay plane is temporarily hidden while the toolbar is displayed.
The Profile View can also be used online during scanning.
• Draw a Profile line in the image display to define a profile. The intensity curves are shown in a separate
graph (see Fig. 672).
• In the Profile View Option control block you can select the width and type of the profile line. The
color setting is taken from the Graphics View Option control block.
• You can place two markers on the profile line to measure differences in intensities and distances in the
XY plane.
• Tick the Profile in Image Display block to see an intensity graph superimposed on the image (see
Fig. 673).
476
CHAPTER 1 - SYSTEM OPERATION
2
i
2
( Ch 2 )
i
000000-2071-464
6.12

Profile View

This function allows to
− display the intensity distribution of an image
along a straight or curved line,
− show the intensity values in table form and
copy table to clipboard or save as text file,
− show separate profiles for each channel in a
multi-channel image.
The settings of the Dimensions, Display, Player
and Graphics view options control blocks apply.
The additional view-specific Profile View Option
LSM 880
10/2014 V_01