Processing - Ion Concentration - Zeiss LSM 880 Operating Manual

LSM 880
5.4.9 Processing – Ion Concentration
standard  optional
Tool accepts single-channel or multi-channel, multi-dimensional data (typically time
series data) of samples labeled with fluorescent ion indicators. Ion Concentration was
developed for analyzing LSM data. Successful analysis of image data obtained with
other systems is potentially possible as well (provided the input meets the
requirements for intensity quantification). See full description of tool for details.
This Processing function permits the calibration of ion
concentrations in physiological experiments.
To open the Ion Concentration tools click Ion
concentration (Fig. 414).
The two buttons select the active image in the
Image Display as Input Image and Input Image 2 (for
background subtraction). The name of the automatically
generated Output Image is shown under the name of
the Input Images.
In the Preview panel at the bottom of the Ion
concentration tool window, thumbnails of the input
images as well as a preview of the output image are
displayed (Fig. 415).
After opening the Method Parameters panel full
window by clicking the little arrow, the full tool window
opens and the following functions are available
(Fig. 416).
buttons
tick box
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Opens the display for the resulting calibration curve.
To load & save calibration curves from / to files.
To select Input Image 2 as background image.
000000-2071-464
Instrument
 LSM ELYRA
Fig. 414
Fig. 415
Lightsheet Z.1
Processing – Ion
concentration
Ion concentration panel
ZEISS
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