Zeiss LSM800-02 Quick Start Quide

Zeiss LSM800-02 + Airyscan confocal microscope
System description:
I. Upright confocal microscope ZEN blue 2.6 HotFix-8
II. 2 GaAsP +1 AiryScan detector (GaAsP)
III. Motorized stage for multiple position imaging
IV. Heating chamber
V. NO CO mixer
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VI. Multi-slide holder, dish holder, single slide holder
Objectives:
- Position1: Objective Plan-Apochromat
- Position2:
Objective Plan-Apochromat
- Position3: Objective Plan-Apochromat
- Position4: Objective
- Objective Plan-Apochromat
- Objective Plan-Apochromat 40x / NA 1.2 Water (not mounted)

Laser lines: 405, 488, 561, 640 nm
Detectors:

Two very sensitive Gallium arsenide phosphide photomultiplier tube (GaAsP PMTs) detectors
with free choice of the spectral range. (Uses a tunable short pass and band pass filter in front
of the PMTs)

Be very careful with using it to prevent instrument damage! If oversaturated, detectors will
switch off automatically.

1 GaASP PMT for the
mode (detection range adjustable by a tunable short pass and a tunable long pass filter, no
bandpass filters)

An ESID detector for transmitted light.
Simultaneous detection of 3 dyes possible.
VII. Starting up:
1. Switch on the multiple socket (1)
to the computer and microscope controllers
10x / NA 0.45 Air
20x / NA 1.0 W
20x / NA 0.8 Air
Plan-Apochromat 40x/1.4 Oil DIC
63x / NA 1.4 Oil
Airyscan detector that can function as a normal detector in confocal
"Hanging on the right side of the
(WD=2.1mm)
DIC (WD=1.8mm)
(WD=0.13 mm)
(not mounted)
20/11/2019
rack". Provides power
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