Zeiss LSM 880 Operating Manual page 176

ZEISS
Fig. 251 Channel color selection
The beam path in the Imaging Setup tool now displays the way the potential emission signal takes.
Channel 1 receives the red part of the spectrum. The settings are suitable for imaging dyes like
Rhodamin, TRITC or Cy3.
For overlaying fluorescence and transmitted-light images, click on the T-PMT (Transmission) button in the
Beam Path and Channel Assignment panel.
All transmitted light applications like
phase contrast
−
differential interference contrast (DIC)
−
polarization contrast (Pol)
−
darkfield
−
can be performed if the hardware of the microscope is equipped with the necessary optics. We
recommend DIC as standard contrast because the suitable objectives have a higher transmission than the
ones for phase contrast. This enhances detection sensitivity.
In case intensity fluctuations occur e.g. with 405 nm lasers, a different attenuation level should be
chosen. In case this is not possible, a different laser line needs to be selected for the T-PMT acquisition or
track.
170
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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• Activate channel 1 via the check box. You may
want to assign a different color to the channel
which will be taken over for the initial display of
the image. Click the color triangle and select
the desired color from the drop down menu
(Fig. 251).
• Use the dye triangle to display the emission
spectrum of your dye.
• Use the slider for the respective channel
(Fig. 249) to set the required detection band
(e.g. 566-644nm).
• If required, deactivate all the other channels
(ChS, Ch2, transmission, R1-2) via check box.
• Click on the icon of the main dichroic beam
splitter and select MBS 488/561 or any other
MBS indicating the 561 line.
LSM 880
10/2014 V_01