Zeiss LSM 880 Operating Manual page 363

LSM 880
Using the channel alignment tool for drift corection
The following procedure can be used to remove lateral
drift in an inage:
• Duplicate the time series. Select the time series in the
processing tool
Duplication (Fig. 507). Press Apply to obtain a copy
from your time series.
• Generate reference image. Using the Processing tool
Copy select the duplicate time series in the submenu
Subset. Using Time select your reference image, for
example the first image or the center image, by
choosing for the Start and End value the same
number (Fig. 508). Press Apply to extract that image
from the time series.
• Generate reference time series. In the Proceesing tool
Modify choose under Modify mode the entry Make
time series. Select the exctracted image. Enter under
Number of times and Time intervals the numbers
from your original time series (Fig. 509) and press
Apply. The result will be a time series with the same
image at each time point.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
Copy under the
000000-2071-464
submenu
Fig. 507
Fig. 508
Fig. 509
Methods Parameter of the
Copy Duplicat Processing tool
Methods Parameter of the
Copy Subset Processing tool
Methods Parameter
Make time series selected in
Modify mode
ZEISS
with
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