Zeiss LSM 880 Operating Manual page 252

ZEISS
Fig. 328 Pixel Depth, Scan Direction & Scan
Average window
Fig. 329 Image of a line before pixel shift
compensation in x
Fig. 330 Image of a line after pixel shift
compensation in x
246
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
000000-2071-464
To compensate for scan zoom and scan speed go
to the Acquisition Mode too of the LSM Online
Acquisition tool group. Use a sample with high
contrast, like "Convolaria", and focus. Activate
Bidirectional Scan
Direction (Fig. 328). During scanning press the
Auto button. The system will adjust automatically
the Scan Corr X and Y values. Alternatively you
can do the compensation manually.
If the Scan Corr is not adjusted, you will see a
pixel shift (Fig. 329). Alter the Scan Corr values to
compensate any pixel shift in the following way:
Move the Scan Corr X slider until the pixel shift is
at the minimum (Fig. 330). Rotate the image by
90° and redo the same adjustment for Scan
Corr Y.
LSM 880
(double arrow) under
10/2014 V_01