Zeiss LSM 880 Operating Manual page 6

ZEISS
• The functions Crop, Split, Delete and Copy used in the file browser menu requires some time for GUI
update.
• When using the channel unmixing function for Airyscan data, the histogram of the resulting image still
refers to the Airyscan data set.
• Airyscan datasets with step sizes too small (much too high oversampling like with step sizes of 10 nm)
lead to faulty processing. Choose 100 nm steps or above as suggested by software for Z-Stack
acquisition.
• When analyzing PALM data for particle tracking, it is possible to select an entry in the table to
highlight the linked particle. It does not work the other way round.
• Do not use PALM image that has already been processed, again as input image for PALM processing.
This leads to GUI artefacts that are only reversible with starting ZEN again.
• When performing Z-Stacks which require averaging of frames, use Line Average. Frame Average can
lead to empty image frames.
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IMPORTANT NOTES
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LSM 880
10/2014 V_01