Multidimensional Acquisition Tools - Positions - Zeiss LSM 880 Operating Manual

ZEISS
5.3.10 Multidimensional Acquisition Tools – Positions
In the Positions tool you can select the carrier and the sample or laser position by the stage or by the
scanners.
• Click on the Positions tool tab to open the Positions tool (Fig. 318). Click again to close the tool.
If no carrier was adjusted in the Carrier Position tool, no carrier will be displayed. You can omit
the adjustment if you work with cells in a single chamber.
Carriers will be schematically displayed with rows indicated by capital Arabic letters and columns by
numbers (Fig. 321).
Fig. 318 Current Positions panel
• The current x and y positions are displayed in the x-Position and y-Position input boxes. You can use
the input boxes to change the position by typing in a number or using the scroll arrows. The next click
on one of the compass rose arrows will bring the stage or to that position. The next click will move the
stage or scanner with the current speed and step sizes in the indicated direction.
If well positions are changed in Sample Carrier the system will automatically activate the XY
Stage. The Sample Carrier tab will only be available for scanning stages. It is deactivated for
manual stages. Even if the Scanner button was activated in Position List, going to Sample
Carrier will automatically deactivate the Scanner and activates the X,Y-Stage mode. If you take
an image of the well than the position can be changed in Scanner mode within the well.
When the required chamber is approached by joystick or the compass rose in Current Position,
orientation of the carrier will be lost. Thus positioning has to be done visually via inspection of the
carrier in this case.
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Stage control (only available if Show all is
activated)
• Activate the XY Stage tab to activate stage or
scan mirror positioning.
• Move the stage in the appropriate position by
clicking on the arrow keys of the Compass
Rose. Each mouse click moves the microscope
stage in the appropriate direction by one step.
• Set the required step width via the Step-size
(µm) the input box or by using its scroll arrows.
The travel speed of the microscope stage can be
set to steps 1, 2 and 3. Step 2 is preferably used
for standard positioning. Step 1 (slow speed)
should only be used for very precise positioning,
since positioning for longer paths requires more
time. Step 3 is suitable if large vessels are used,
since precise center positioning only plays a
minor role in such cases.
LSM 880
10/2014 V_01