Zeiss LSM 880 Operating Manual page 493

LSM 880 Center Screen Area / Image Containers - Display and Image Analysis
Available tools in the FRAP View:
Channels
Kinetic Model / Fit
formula
Analyis Areas /
Regions of Interest
(ROIs)
Background
Region
Reference Region
Combine Regions
Group 1, 2, 3
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CHAPTER 1 - SYSTEM OPERATION
Select single channels or all channels for analysis in the general Dimensions view
options control box.
Pull down menu to select the mathematical model (mono or double exponential
model) for fitting the data.
The FRAP View Options control block includes drawing tools that work
identically to the graphics view options control block. ROIs from both control
blocks can be combined.
Check box in the list of ROIs: Mark the region of interest which represents the
mean background intensity to be used for data correction.
Check box in the list of ROIs: Choose and mark the ROI which represents the
fluorescence intensity of a reference cell that has not been bleached. The mean
intensity within that region is used to correct the data at each time point for any
bleaching artifact that occurred during the imaging process.
Check boxes in the list of ROIs which allow to choose more than one ROI for
analysis and to group them according to the experimental set up.
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