Zeiss LSM 880 Operating Manual page 115

LSM 880
In the Sum method, the intensity values of all images are added up, without a mean value being
calculated.
To create the image information using the Line average mode, each line (depending on the setting) is
scanned 2, 4, 8 or 16 times during Scan Average, and then the average value per pixel is calculated. This
minimizes noise interference during the scanning procedure.
If the Frame average mode is used to create the image information, the complete frame is scanned 2, 4,
8 or 16 times, depending on the setting. The average value is recalculated after each frame scan.
The Frame average mode also permits continuous averaging.
For this, select the Continuous option in the Number selection box.
Scan Area
In this panel, the scan field is set for zoom, rotation
and offset in relation to the field of view of the
microscope. The diagonals of the outer square on
the left-hand side correspond to the field of view
of the microscope.
The inner square contained in it (rectangle in the
case of differently set frame size) represents the
scan range and immediately shows the changes
made to zoom, rotation and offset.
• Set the desired Zoom factor via the slider or by clicking on the small red squares outlining the inner
square.
The Zoom factor can be set continuously in the range from 0.6 (0.7 for Axio Examiner stand) to the
−
maximum of 40, and is displayed in the relevant input box. The value 0.6 corresponds to factor 1,
and value 40 to factor 66, related to the field of view. From zoom factor 5 onwards, the
magnification will be empty.
Recommended setting to start with: Zoom 1.
−
• To rotate the scan area, use the slider or click on the blue dot in one of the corners of the inner
square.
− Recommended setting to start with: Rotation 0°.
• Move the scan area by clicking on
and
• The offset of the scan area from the center of the field of view is displayed online in µm for X and Y.
Clicking, holding and drawing the rectangle with the mouse permits the scan area to be moved
−
directly within the field of view.
Recommended setting to start with: Offset X = 0, Y = 0
−
During the scan procedure, the functions Speed, Scan Corr, Zoom, Rotation and Offset can
be influenced online.
By clicking on the Reset All button the scan zoom is set to 1, the XY offsets are set to the zero position
and the ratio angle is set to 0°.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
for offset in vertical direction.
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Fig. 136 Scan Area panel
for offset in horizontal direction
ZEISS
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