Zeiss LSM 880 Operating Manual page 199

LSM 880
− Upper Limit: defines the upper limit value tolerated from a fit; if this value is exceeded, the fit is
rejected and another (global) minimum is searched for. The default value depends on the
parameter. (Only available if the Limits... button is pressed that will open the Fit parameter
window (Fig. 273)).
− Link: links the parameter globally. Type M, K, P, R and C to link the parameter over measurements,
kinetic indices, positions, repetitions and channels, respectively.
• The Status display window gives information
on conflicts in the entries. For example, if the
structural parameter was fixed to 5, but its
lower limit set to 6, the system will report this
conflict.
• Before saving you can return to the old default
settings by pressing the Reset button. This
button is also
parameters window, which can be opened by
pressing the Limits... button and closed by
pressing its Close button. Reset will undo all
changes. If the method was saved, the new
entries will be the default.
Generally it is accepted that non-linear fitting
procedures yield more reliable results when
the number of free floating parameters is
low. It is recommended to fix parameters
which are known
measurements. Good candidates for fixing
are diffusion times of the free dye and the
free (i.e. not bound) partner, which can be
determined in
Another good candidate is the structural
parameter that is an instrumental parameter.
If you save the method all changes will be
saved with the methods.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
available from the
from independent
previous measurements.
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Fit
Fig. 273 Fit parameter window
ZEISS
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