Zeiss LSM 880 Operating Manual page 220

ZEISS
Fig. 277 Background Settings panel
Fig. 278 Amplitude Settings panel
214
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Background
• You can set an offset by setting a value in the
Background selection box (Fig. 277). Either
type in a value or use the arrows.
The description box gives you information on the
background.
If there is no background selected, the back-
ground correction factor will be set to 1.
Amplitude
• You can set a value for the geometric factor γ,
which describes the point-spread function
(Fig. 278).
• By pressing the cylindrical, 2DG, 3DG and GL
buttons you can
dimensional Gaussian, 3 dimensional Gaussian
(3DG) and Gaussian-Lorentzian
respectively. If you press the Calibrate button,
you can type in a user defined number in
selection box or use the arrows to set a value.
The description box gives you information on the
amplitude.
At least one the γ factor or the number of
molecules N have to be fixed in the fit
procedure.
LSM 880
select a cylindrical,
(GL) PSF,
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