Interpolate Brightness And Contrast - Zeiss LSM 880 Operating Manual

ZEISS
Fig. 548 Processing – Adjust with
Interpolate brightness and
contrast
Fig. 549 Processing – Adjust with
Interpolate brightness and
contrast tool with loaded
Z-Stack
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
5.4.22.2
This function permits a continuous contrast and
brightness adjustment in a Z-Stack or Z-Stacks over
time by interpolation between the starting and end
values of the respective image series. This permits a
post-acquisition compensation of signal loss in, for
example, thick tissue imaging where excitation and
detection
Interpolation can be defined for the entire image or
only for individual channels. In addition, a set of
coordinates for which intensities are kept fixed during
the interpolation calculation can be defined (Fig. 548).
The Select button selects the active image in the
Image Display as the Input Image to be processed.
In the Interpolation panel (Fig. 549), the parameters
for the interpolation procedure are set.
• In the Coordinate pull down, select the dimension
• Use the List size spin box to set the number of
• Highlight a row of your choice by clicking on it.
• Use the available All, Ch1-T1 and Ch2-T2 channel
• Having set the parameters, click on the Apply
• The newly created image (series) can be stored
000000-2071-464

Interpolate Brightness and Contrast

efficiency
for the interpolation. Depending on the image
data type, x, y, z, and time are the possible
options.
fixed data points. The table in the Interpolation
panel will be extended to the selected number of
rows.
Then use the Brightness, Contrast and Z (or t)
slider to set the values for this fixed data point. The
numbers in the table will be updated according to
the slider position. Then highlight the next row, set
the parameters and keep setting the fixed data
points for all rows.
buttons to select the channel for interpolation or
click on the All button if the entire image is to be
interpolated.
button. Interpolation will be performed in a new
Image Display window.
using the Save As function.
If you de-activate the Extrapolate check box,
only the slices lying between the first and last
fixed data point (table rows) will be taken into
account for interpolation. Otherwise, brightness
and contrast will also be changed for the other
slices.
LSM 880
decrease significantly.
10/2014 V_01