Zeiss LSM 880 Operating Manual page 157

LSM 880
If a region is used to define a position for bleaching within the sample, the bleach process can be
accelerated by checking the box next to Zoom Bleach (fast, less accurate). In this case the scanner
movement will be restricted to the bleach region zooming in onto this region. This may result in a less
accurate positioning of the region as the definition of the region has been made in a different zoom in
the image. The bleach process will be faster than without this option. The gain in speed is dependent on
the speed that is used for bleaching. If a high speed has already been chosen for bleaching the gain in
speed will be minimal.
Define the region(s) for bleaching in the Regions tool (see section Acquisition Parameter – Regions).
Only a region activated under Bleach is considered for bleaching. In the Excitation of Bleach area
(Fig. 202) choose the laser line and laser intensity which is then used for bleaching.
It is possible to choose different laser lines and/or laser intensities for the bleach process of each region
when activating the option: Use different Settings for different ROIs. A maximum number of six
regions is possible. The numbers of the regions correspond to the numbers indicated next to ROI#. For
each region, a tab is active which allows setting individual laser parameters for bleaching.
Define the region(s) for bleaching in the Regions
tool. Only a region activated under Bleach is
considered for bleaching. In the Excitation of
Bleach area (Fig. 203) chose the laser line and
laser intensity which is then used for bleaching.
It is possible to choose different laser lines and/or
laser intensities for the bleach process of each
region when activating the option: Use different
Settings for different ROIs. The numbers of the
regions correspond to the numbers indicated next
to ROI#. For each region, a tab is active which
allows setting individual laser parameters for
bleaching.
The Regions tool is only available in the LSM
imaging mode.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Fig. 203 Excitation of Bleach area for setting
laser line
bleaching
ZEISS
and intensity for
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