Related Manuals for Zeiss LSM900
Summary of Contents for Zeiss LSM900
- Page 1 Manual Zeiss LSM 900 Zeiss LSM900 Room Be311 Erasmus Optical Imaging Centre document version #1...
- Page 2 Manual Zeiss LSM 900 A few points of attention: - Do not use too much oil - Do not use oil on the dry-lenses - Clean the oil-lenses (40X, 63X) after use with a tissue and ethanol - Do not eat or drink behind the microscope...
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Page 3: Turning On The System
Manual Zeiss LSM 900 Turning on the system 1. Turn on the ‘system’-switch behind the table, box above the box with the key. DO NOT TOUCH OTHER SWITCHES OR THE KEY! 2. Turn on the connector box on the microscope-table. DO NOT TURN ON OR OFF THE MICROSCOPE. - Page 4 Manual Zeiss LSM 900 Making a new configuration and a quick first confocal image Tip: undock the channels window by clicking on the arrow pointing up to the right 1. Click ‘acquisition’ and pull the slider on the microscope totally out (right side of the oculars on the microscope) 2.
- Page 5 Manual Zeiss LSM 900 Zooming in 1. In the ‘Acquisition Mode’-window click on ‘Crop Area’: a square will appear on screen 2. Click on the little cross, hold and move the square over the object of interest 3. The square is already zoom 2. You can increase the zoom by clicking on a corner, hold it and move the mouse so a smaller square will be made.
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Page 6: Saving The Image
Manual Zeiss LSM 900 Saving the image There are 2 ways of saving your image in .CZI format: uncompressed or compressed Uncompressed: 1. Click ‘File’ and choose ‘Save as CZI’ 2. Go to the right network-folder, give your image a name and press ‘Save’... - Page 7 Manual Zeiss LSM 900 Tile module See Picture A next to microscope for yellow numbers Making an overview: Decide if you want to make an overview. If not: go to Tile-scan with high magnification 1. Decide if you want to make an overview with the 5X-lens (mostly used to see where tissue or...
- Page 8 Manual Zeiss LSM 900 A preview will be made and showing on the screen. The preview image can be saved by right-clicking on the image and choose ‘Save preview images’: the image will appear in the main window and can be saved with ‘Save as CZI’.
- Page 9 Manual Zeiss LSM 900 The result images can also be reloaded in the tile-viewer in a later stage, even on another day. Be sure to put the right slide in the same way under the microscope! Images with more scenes can be split into separate images at ‘Processing’-‘Utilities’-‘Split Scenes’, also possible with the freeware Zen-software you can install on your own computer.
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Page 10: Shutting Down The System
Manual Zeiss LSM 900 14. Open the ‘Software autofocus’ window 15. Activate ‘Intensity’, ‘Smart’, ‘Default’, ‘Relative Range’ and check on ‘Automatic Range’ Now you can image as before: 16. Check on ‘Automation’ (15) 17. In the automation-window check on ‘Run macro after experiment execution’, click on the dots and choose ’01...