Zeiss LSM 880 Operating Manual page 477

LSM 880 Center Screen Area / Image Containers - Display and Image Analysis
Histogram functions:
Tables can always be saved or copied to the clipboard by right-mouse clicking on the table
display!
Histogram functions and Overlays:
The functionality of the Graphics View Option
control block fully applies to the Histo View Type.
If a ROI is marked/selected in the Histo View Image
display, only this ROI will be taken into account for
the histogram display, the table and the statistics.
If there are several
marked/selected, the histogram display, the table
and the statistics analyze all the pixels included in
all ROIs.
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CHAPTER 1 - SYSTEM OPERATION
Skip Black and Skip White tick boxes: Ignore black pixels (gray value 0) and
Ignore white pixels (gray value 255 or 4096) in the histogram.
Step slider and spin box: Sets the number of intensity steps ("trays") of the
histogram display. Step 1 corresponds to 256 intensity steps, Step 64 to 4
intensity steps (for 8 bit images). Reduction is made by averaging.
Scaling pull down menu: Sets the histogram graph scaling to either dynamic
(adjusting to the maximum value) or fixed
Show Table tick box: A table of the intensity values is shown in the Image
Display window.
Show Statistics tick box: Displays statistical parameters (Mean Intensity,
Standard Deviation, Number of Pixels and Size of Area) in an additional
table. Area measurements of very small areas (<10 pixels) give only
approximate values.
Show Image tick box: Shows the image in the Image Display window with
the histogram graph.
The Cut Mask tool creates a new image document which sets every pixel
outside ROIs to Zero. Within the ROIs, only the pixels with values between
lower and upper threshold are transferred to the new image.
ROIs, but none
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Fig. 667 Histo View Option control block -
Threshold
is
ZEISS
471