Zeiss LSM 880 Operating Manual page 141

LSM 880
Threshold: High – Low: A low threshold for marker detection is used when the dimensions of the
correlative L markers cannot be recognized precisely, e.g. when the sample holder is slightly filthy.
Marker Color: White – Black – Automatic: Select the color of the markers (as it appears in the image
window) or leave it to automatic.
White: The marker is displayed white on a dark background.
Black: The marker is displayed dark on light background.
Automatic: The marker color is set automatically.
Marker Orientation: Select the correct orientation of the L-markers on your sample holder from the
Marker Orientation field. Click on the corresponding button to set the same orientation like you see it
in the live image.
Sample Holder Calibration field: The marker recognition during the calibration can be done either
automatically or manually. During the calibration the stage can moved automatically to the next marker,
when the Automatic movement to next marker checkbox is activated. If the stage is moving in the
wrong direction, invert the direction by clicking on X Invert or Y Invert in the Sample Holder
Calibration field.
If the first marker position was already set once and x/y coordinates are given, the stage can simply be
moved to marker position one by clicking on the Position 1 button.
a) Automatic marker recognition
The software proposes the position of the marker
visible in the live image.
• Click on the Continuous button to start
continuous scanning.
• Move the stage to the first marker manually or
click Position 1 in the software in case the first
marker position was already set once and x/y
coordinates are given.
• Click
Automatic
(Fig. 179).
The software will propose the position of marker 1
in a separate window (Marker location found
dialog (Fig. 180)).
• Click on Accept in the Marker location found
dialog to confirm the proposed marker position.
Click Reject if proposed position is not correct.
Contamination of the markers or weak
contrast settings may interfere with the
marker recognition.
If the proposed position is not correct,
either optimize the contrast settings or
use the manual marker recognition.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
in the Position 1 field
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Fig. 179 Position 1 field
Fig. 180 Marker location found dialog
ZEISS
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