Zeiss LSM 880 Operating Manual page 214

ZEISS
t
r
−
1
⋅ ⋅
R ( c e
t
= +
a 1
G ( ) 1
r
1
with R being the amplitude, c
a
rotational diffusion times.
, t and t
R are fitted parameters; c
a r,1 r,2
defined.
Rotational frequencies take often the following values:
r 1
1
r 10/3
2
The relative amplitudes dependent on the polarization of the excitation light and the analyzer in the
emission beam path and are as follows:
Ex lin. lin. pol.
pol.
Em parallel perpendicular all
c 80 20/9
1
c 64/9 4
2
208
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
t
r
−
2
t t
+ ⋅
r 1 , c e r 2 , )
normalized
2
−
R
a
and c relative amplitudes, r
1 2
and c
1
lin. pol. unpol.
parallel perpendicular all
860/9 5/9
4 16/9
000000-2071-464
frequency factors and t
and r
1 2
as well as r and r
2 1 2
unpol. unpol.
215/9 20
1 1
are fixed values and must be user
10/2014 V_01
LSM 880
(8f)
and t
the
r,1 r,2