Channel Shift - Zeiss LSM 880 Operating Manual

LSM 880
5.4.22.3

Channel Shift

Channel shift is used to produce a congruent
image with relation to the pixels of the various
channels (Fig. 550).
This pixel correction function is particularly
important in UV applications.
This is a manipulation of your data that has
to be considered very carefully when co-
localization analysis
experiment.
The Select button selects the active image in the
Image Display as the Input Image.
The image has to be a multi-channel image.
• Select the channels you want to shift by ticking
the Ch1-T1 or Ch2-T2 check box:
• Use the X and Y sliders or spin boxes to define
the shift in pixels to select the pixel shift in the
horizontal and vertical direction. Clicking the
Zero next to the spin-boxes resets the shift to
the original position.
A preview is automatically generated at the
bottom of the Channel Shift tool in the
Processing tab of the Left Tool Area At the same
time, a large preview is shown in the Preview
view tab in the Image display in the Center Screen
Area.
• Click on the Apply button to generate a new
image with the set pixel shift.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
is part of your
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Fig. 550 Processing – Adjust with Channel
shift
ZEISS
393