Zeiss LSM 880 Operating Manual page 64

ZEISS
Fig. 58 Axio Observer.Z1 controls in the
Microscope Control tool
(2) Microscope settings on Axio Observer for transmitted-light observation
• Click on the Transmitted light button. Click the On button in the Transmitted Light panel and set
the transmitted light intensity via the slider or click on 3200 K.
• Click on the Condenser button and set the aperture via the slider in the Condenser panel. Set the
filter in the Filter selection box.
• Click on the Objective button and select the objective by clicking on it.
• Click on the Reflector button and select None.
• Make sure there is no illumination from the reflected-light.
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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(1) Conventional setting of the microscope
Axio Observer.Z1
The Recording of microscope settings works as
described for the microscope Axio Imager.
For the conventional
Axio Observer.Z1, proceed as follows:
• Click the Locate tab in the Main toolbar.
• Place specimen on microscope stage.
− The cover slip must be facing down.
• In the Objective list box, select the required
objective.
• Use the focusing drive (Fig. 59/4) to focus the
required specimen plane.
• Select specimen detail by moving the stage in
X and Y via the XY stage fine motion control
(Fig. 59/3 and 2).
LSM 880
setting of the
10/2014 V_01