How To Optimize Imaging With Airyscan - Zeiss LSM 880 Operating Manual

LSM 880
A similar procedure applies for image optimization if more than one channel is used and simultaneous
image acquisition is performed.
Make sure the suitable lasers are switched on and the channels are activated.
Pay attention to address the right channel when pinhole size, detector gain, and amplifier offset are
controlled as these are set individually for each channel.
Use the split view (see section Split View) to see the effect of the controls onto each channel separately.
If the acquisition is performed sequentially in with multiple tracks it is faster to activate only one track at a
time for image optimization.
5.2.23.5 How to Optimize Imaging with
Airyscan
Laser, Gain (Fig. 256)
For correct results with the Airyscan detection and
processing, overexposure needs absolutely to be
avoided.
By modifying
Laser power
−
and detection Gain
−
the detection can be set to the required level.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Fig. 256 Optimize imaging with Airyscan
ZEISS
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