Zeiss LSM 880 NLO Manuals

Manuals and User Guides for Zeiss LSM 880 NLO. We have 2 Zeiss LSM 880 NLO manuals available for free PDF download: Operating Manual, Notes On Device Safety And Installation Requirements

Zeiss LSM 880 NLO Operating Manual

Zeiss LSM 880 NLO Operating Manual (690 pages)

Brand: Zeiss | Category: Microscope | Size: 18.18 MB

Table of Contents

Chapter 1 System Operation

7
- Table of Contents
  7
- Convention for the Text in this Manual
  14
- Purpose
  14
- Software
  14
- Backup
  15
- Software Operation
  15
- Hardware Aspects
  16
- Principle of Laser Scanning Microscopy
  16
- Principle of Fluorescence Correlation Spectroscopy
  17
- Principle of Airyscanning
  18
- Optical Diagram of the LSM 880 (Schematic)
  19
- Performance Features of the LSM 880
  20
- Microscope Equipment of the LSM 880
  21
- Computer Hardware and Software
  23
- Startup and Shutdown of the System
  24
- Startup of the System
  24
- The Power Remote Switch
  24
  - Additional Software Tools
    25
- Starting ZEN
  26
- Exiting ZEN Software
  29
- Shutdown Procedure
  29
- Switching System Power off
  31
- Introduction to the Software Application Layout
  32
- Overview on the Screen Layout
  32
- Introduction to ZEN
  33
- Function Elements
  36
- Application Bar
  38
- Menu Bar
  38
- File
  40
- Maintain
  41
  - Set Spline
    41
  - Camera
    42
  - Hardware Administrator
    42
  - Test Grid
    42
- Help - about
  43
- Main Toolbar
  44
- Status Bar
  46
- Left Tool Area
  47
- Tool Groups and Tools
  49
- Context Menu of the Left Tool Area
  50
- Center Screen Area
  51
- Overview of the Center Screen Area
  51
- Context Menu of the Center Screen Area
  52
- Right Tool Area
  54
- Left Tool Area and Hardware Control Tools
  56
- Locate Tab
  57
- Microscope Control Tool for Axio Observer.z1
  59
- Microscope Control Tool for Axio Imager.z2
  66
- Controls for Axio Examiner
  69
- Incubation Tool
  70
- Acquisition Tab
  71
- Configuration Functions
  72
- Smart Setup
  74
- Action Buttons
  79
- Multidimensional Acquisition Settings
  81
- Setup Manager - Laser
  83
- Setup Manager - Experiment Designer
  86
- Setup Manager - Imaging Setup
  90
  - Imaging Mode and Track Definition
    90
  - Filter Cube Selection
    92
  - Objective Selection
    92
  - Stage Control
    93
  - Focus Control
    94
  - HAL Control
    94
  - X-Cite Control
    95
  - Aperture Control
    95
  - Ratio Imaging
    96
  - Laser Control
    96
  - Control Elements of the Imaging Setup Tool
    97
  - Setup Imaging with Airyscan Detector
    105
- Acquisition Parameter - Acquisition Mode
  107
  - Objective Selection
    107
  - Scan Mode
    108
  - Format Selection
    108
  - Averaging and Color Depth Selection
    109
  - Hdr(High Dynamic Range)-Imaging
    110
  - Scan Area Definition
    111
  - Acquisition Mode Functions for LSM
    112
  - Acquisition Mode Settings for Airyscan
    119
- Acquisition Parameter - Channels
  121
- Acquisition Parameter - Focus
  132
- Acquisition Parameter - Focus Devices and Strategy
  133
- Acquisition Parameter - Stage
  137
- Acquisition Parameter - Shuttle and Find
  138
- Acquisition Parameter - Regions
  144
- Multidimensional Acquisition - Z-Stack
  147
  - Performing a Z-Stack Using First/Last Mode
    148
  - Performing a Z-Stack Using Center Mode
    150
  - Performing a Z-Stack Using a Z-Piezo
    152
  - Optimize Sectioning and Step
    153
  - Correction
    154
  - Z-Stack Settings for Airyscan
    155
- Multidimensional Acquisition - Bleaching
  156
- Multidimensional Acquisition - Tile Scan
  158
- Scan Overview Image for Tiling
  160
- Multidimensional Acquisition - Time Series
  161
  - Interval Time
    161
  - Marker
    163
  - Start
    165
  - End
    167
- Multidimensional Acquisition - Positions
  168
  - Position List
    168
  - Sample Carrier
    169
  - Setting Individual Offset Values for Positions in Position List Mode
    172
  - Lowering Objective When Moving the Stage
    172
  - Scan Overview Image
    173
- Multidimensional Acquisition - Information on Experiment
  173
- Multidimensional Acquisition - Auto Save
  174
- Image Optimization
  175
  - Requirements
    175
  - Defining the Scan Parameters
    177
  - Defining the Detection Parameters
    177
  - How to Optimize Image Quality
    178
  - How to Optimize Imaging with Airyscan
    179
- FCS Tab
  181
- Setup - Laser
  183
- Setup - Light Path
  183
  - Light Path for 2 Channel Module (Big.2 on DC out Port)
    185
  - NDD Light Path (Big.2 as NDD)
    187
  - Light Path for 32 Channel Module
    189
  - Selection of Laser Lines
    190
  - Pinhole Adjustment
    192
- Setup - Acquisition
  196
- Setup - Fit
  198
  - Defining a New Model
    200
  - Correlation Options
    201
  - Model Equations
    202
  - Definition of a Model
    219
  - Pch
    228
  - Formula
    229
- Setup - Correlator
  230
- Navigation - Stage
  234
- Navigation - Sample Carrier
  235
- Navigation - Focus
  237
- Multidimensional Acquisition Tools - Time Series
  242
- Multidimensional Acquisition Tools - Positions
  244
- Navigation - Incubation
  253
- Multidimensional Acquisition Tools - Information on Experiment
  254
- Action Buttons for FCS
  255
- Method Tool
  256
- Activation Tab Area
  259
- New
  259
- Count Rate
  260
- X-Y-Z Scan
  262
- Snap
  265
- Start Experiment
  265
- Taking FCS Measurements
  266
  - Start and End of a Measurement
    269
  - Loading Stored Data Files
    273
  - FCS Correlation Diagram
    274
  - Image View Controls - Correlation Tab
    277
  - Image View Controls - Table Tab
    279
  - Saving Correlation Results
    284
  - Coincidence
    286
  - Fit
    287
  - Print
    296
  - 5.3.21.10 Information
    298
- General Structure of the Processing Tab
  299
- Processing Tab
  299
- Processing - Airyscan Processing
  303
- Processing - Maximum Intensity Projection
  306
- Processing - Color-Coded Projection
  307
- Processing - Image Calculator
  308
- Processing - Average
  310
- Processing - Filter
  311
- Processing - Linear Unmixing
  314
- Processing - Ion Concentration
  319
  - Single Wavelength Dyes - Offline Calibration
    321
  - Ratiometric Dyes
    322
- Processing - Correlation
  325
- Processing - Modify Series
  326
- Processing - HDR-Imaging
  327
- Processing - Stitch
  331
- Processing - PALM
  332
  - PALM - Convert to Image
    333
  - PALM - Import PALM Molecules
    334
  - PALM - Localization Precision
    335
  - Palm - Palm
    336
  - PALM - Remove PALM Outliers
    344
- Processing - Particle Tracking
  345
- Processing - Experimental PSF
  347
- Processing - Structured Illumination
  351
- Processing - Channel Alignment
  360
- Processing - Lightsheet Processing
  367
  - Dual Side Fusion
    367
  - Multiview Processing
    370
  - Online Multiview Processing
    387
- Processing - Copy
  388
- Processing - ICS
  390
  - Detrend
    390
  - Remove Structure
    391
  - ICS Correlation
    392
  - Map Filter
    393
  - Nandb Analysis
    394
- Processing - Adjust
  397
  - Burn in Brightness and Contrast
    397
  - Interpolate Brightness and Contrast
    398
  - Channel Shift
    399
  - Shading Correction
    400
- Maintain Tab
  401
- Maintain - Adjust Pinhole and Collimators
  402
  - Pinholes Section Panel
    403
  - Collimators Section Panel
    404
  - How to Adjust the Pinhole and Collimator
    405
- Maintain - Objectives
  408
  - Objective
    408
  - Select Objective
    409
  - Add Objective
    410
  - Remove Objective
    411
  - Show/Edit Objective
    411
  - Focus Speed
    412
  - Parfocal Correction
    412
- Maintain - Filters
  413
- Maintain - Airyscan
  414
- Options - System Options
  415
  - System Options - Load Configurations
    416
  - System Options - Reuse
    416
  - System Options - Hardware
    417
  - System Options - Image Display
    418
- Options - FCS Options
  419
  - FCS Options - Data
    419
  - FCS Options - Measure
    423
- Options - Service
  424
  - FCS Options - Auto Save
    424
- Center Screen Area / Image Containers - Display and Image Analysis
  425
- General Structure
  425
- Structure and Functional Concept of the Center Screen Area and the Image Display Container
  425
- Container Configuration with the Container Context Menu
  429
- Dimensions
  430
- View
  430
- Display
  433
- Player
  434
- Graphics
  435
- Preview View
  438
- Split View
  439
- Gallery View
  442
- Airyscan - View Option Tab Dimensions
  443
- Airyscan View
  443
- Airyscan - View Option Tab Display
  444
- Airyscan - View Option Tab Airyscan
  445
- Ortho View
  449
- Ortho - Select Function
  450
- Ortho - Distance Function
  451
- Cut View
  452
- D View
  453
- View
  455
- Shadow Projection
  457
- Transparency Render Mode
  460
- Maximum Mode
  461
- Surface Render Mode
  461
- Mixed Render Mode
  462
- Clipping Planes
  463
- Flying Mode
  467
- Rendering Settings in Visartplus
  467
- Series
  469
- Interactive Measurements
  471
- Settings
  472
- Options
  473
- View - Basic
  474
- Histogram View
  475
- Colocalization View
  478
- How a Scatter Diagram Is Generated
  480
- Colocalization Coefficients
  481
- Quantitative Colocalization Parameters Shown in the Data Table
  481
- Overlap Coefficient, Overlap Coefficient after Manders
  482
- Profile View
  482
- Shuttle and Find View
  485
- Mean of ROI: Additional View Type for Time Series
  488
- Kinetic / FRAP View: Additional View Type for Time Series
  492
- Lambda Coded: Additional View Types for Lambda Mode
  498
- Unmixing View
  500
- Automatic Component Extraction
  505
- FRET View
  507
- Tools in the FRET View Options Control Block for Acceptor Photobleaching
  509
- Tools in the FRET View Options Control Block for Sensitized Emission
  511
- Raster Scanning Image Correlation Spectroscopy (RICS)
  514
- Access to RICS
  515
- Control of View Functionalities
  515
- Correlation Functionalities
  516
- Display Functionalities
  519
- Fitting Functionalities
  526
- Model Functionalities
  529
- Polarization Imaging
  533
- Imaging Setup for Polarization Imaging
  534
- Determination of G-Factor
  535
- Obtaining an Anisotropy Image
  536
- Information View
  538
- General
  540
- Right Tool Area, Data Management and Storage
  540
- ZEN File Browser
  541
- Gallery View of the ZEN File Browser
  544
- Form View of the ZEN File Browser
  546
  - Table View of the ZEN File Browser
    547
- Images and Documents Panel
  548
- Opening of Files Via the "Open" Command in the File Menu
  551
- Save
  551
- Export of Images
  552
- Index
  553
Chapter 2 Macros and Visual Basic

559
- Macro Language
  561
- Macros
  561
- VBA Version
  561
- Edit Macro Function
  562
- Macro Control
  562
- Assign Macro to the Macro List
  565
- Editing and Debugging of Macros
  566
- Overview of Available Macros
  567
- Sample Macros
  568
- Excitationfingerprinting
  569
- Concatenateimages
  572
- Open / Close the Visual Macro Editor Window
  573
- Visual Macro Editor
  573
- Function Description
  574
- Editing a Macro
  581
- Programming for LSM: Starting Points
  584
- VBA Programming
  584
- VBA Programming for LSM
  584
- Object Browser
  585
- Glossary
  586
- Programming with the Lsm5Vba Interface
  586
- Introduction
  588
- Programming with the VBA-GUI Interface
  588
- Recording a Macro
  589
- Structure of a VBA-GUI Command
  589
- Selecting and Managing Image Documents
  590
- Technical Note
  590
- Access to Images
  591
- Backup Recording Settings
  591
- Event Handling
  591
- Example Macros
  591
- Export, Save and Load
  591
- Vba-Gui Api
  592
Chapter 3 Additional Software Tools

593
- Stand Select
  594
- Calibration Objective
  595
- Systemmaintenance Tool
  595
- Starting the Maintenance Tool
  596
- General Operation of the Maintenance Tool
  597
- ZEN Configuration Tool
  599
Chapter 4 Annex

603

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Zeiss LSM 880 NLO Notes On Device Safety And Installation Requirements

Zeiss LSM 880 NLO Notes On Device Safety And Installation Requirements (50 pages)

Brand: Zeiss | Category: Microscope | Size: 4.1 MB

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