Multidimensional Acquisition - Time Series; Interval Time - Zeiss LSM 880 Operating Manual

LSM 880
5.2.19 Multidimensional Acquisition –
Time Series
When the Time Series tool tab is expanded the
Time Series tool becomes available (Fig. 211). In
the Time Series tool the parameters for the
sequential acquisition of image frames (and lines
for the LSM image mode) as time series are
defined. Imaging in spot mode is only possible in
combination with the settings of a time series
acquisition in the LSM imaging mode. The
options in this mode within the Time Series tool
are limited and explained below.
To set up a simple time series acquisition it is enough to define the number of Cycles, which corresponds
to the number of frames (or lines), imaged. This number can be defined with the Cycles slider or the
Cycles input box. A maximum value of 100,000 can be set. The amount of data that can be handled by
the system is limited. If the maximum amount is exceeded a warning will appear. In this case, it is
necessary to either change the destination for data storage and/or reduce the amount of cycles. The limit
also depends on the imaging setup and the acquisition mode. Note that it is an upper limit of handling
data files, but this is beyond the value of 100 Gigabits.
Interval defines a potential time interval, set in milliseconds (ms), seconds (sec) or minutes (min) by the
Interval drop down menu, to separate the beginning of two cycles. A time shorter than the scan time
for a frame (or line) is not effective. The interval time can be changed by using the Interval slider or
Interval input box, also while scanning.
There are different advanced tools (only available in Show all mode) including

− Interval Time

− Marker
− Start
− End
5.2.19.1 Interval Time
Expand the Interval field by clicking the triangle
(Fig. 212). An Interval Time is a potential time
interval between the end of one cycle and the
beginning of the next cycle. The time and time unit
for the delay time is set using the slider or input
box of Interval Time. A cycle interval time is not
effective if a cycle delay time is defined, since the
interval time is always included in the delay. A total
of six different delay times can be defined by
adding further lines clicking on the + button.
Clicking the – button
highlighted delay time. Each delay time can be
controlled using the Trigger In function.
(For a detailed description on the trigger use and interface see printed manual CHAPTER 4: ANNEX).
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
deletes the currently
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Fig. 211 Time Series tool
Fig. 212 Time Series tool – Interval Time
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