Zeiss LSM 880 Operating Manual page 118

ZEISS
If the defined free shape curve becomes too complicated or the selected Scan Speed is too high, the
following message appears in the status bar:
Maximum scanner acceleration exceeded!
• In this case, reduce the Scan Speed set in the Acquisition Mode tool.
• If the generated contour and the line traced by the scanner are not in coincidence, reduce the Scan
Speed by a further amount.
If no sufficient coincidence of the two lines can be achieved by the reduction of the scan speed, you have
to calibrate the scanner position signal.
Fig. 139 Acquisition Mode tool - Spot Mode
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Spot Mode
In the Spot mode fluorescent or reflected light
occurring from a single voxel xyz is detected. In this
mode a spot can be defined by two perpendicular
lines in the Image using the Spot Select button
which is located in the lower part of the
Acquisition Mode tool window (Fig. 139).
The following parameters for scanning a spot can
be defined:
Objective, Speed, Bit Depth and Offset in X
and Y (= position within the image)
These functions operate as described in section
Frame Mode for LSM.
In Spot mode it is not possible to acquire a
Z-Stack. After definition of the spot position the
only possible scan mode is Time Series.
Spot scan data can also be displayed as images
consisting of two lines with 500 pixel per line.
When indicating the number of cycles in the Time
Series tool window, one cycle equals 1000 pixels.
LSM 880
10/2014 V_01