Zeiss LSM 880 Operating Manual page 11

LSM 880
5.4.12 Processing - HDR-imaging ............................................................................................ 321
5.4.13 Processing - Stitch ........................................................................................................ 325
5.4.14 Processing - PALM ........................................................................................................ 326
5.4.14.1 PALM - Convert to Image ............................................................................................. 327
5.4.14.2 PALM - Import PALM Molecules ................................................................................... 328
5.4.14.3 PALM - Localization Precision........................................................................................ 329
5.4.14.4 PALM - PALM .............................................................................................................. 330
5.4.14.5 PALM - Remove PALM Outliers ..................................................................................... 338
5.4.15 Processing - Particle Tracking ........................................................................................ 339
5.4.16 Processing - Experimental PSF ....................................................................................... 341
5.4.17 Processing - Structured Illumination .............................................................................. 345
5.4.17.1 Structured Illumination - Fourier Transform ................................................................... 346
5.4.17.2 Structured Illumination - Structured Illumination............................................................ 347
5.4.17.3 Structured Illumination - TIRF-Filtered ............................................................................ 352
5.4.18 Processing - Channel Alignment ................................................................................... 354
5.4.19 Processing - Lightsheet Processing ................................................................................ 361
5.4.19.1 Dual Side Fusion ........................................................................................................... 361
5.4.19.2 Multiview Processing .................................................................................................... 364
5.4.19.3 Online Multiview Processing .......................................................................................... 381
5.4.20 Processing - Copy ......................................................................................................... 382
5.4.21 Processing - ICS ........................................................................................................... 384
5.4.21.1 Detrend ........................................................................................................................ 384
5.4.21.2 Remove Structure ......................................................................................................... 385
5.4.21.3 ICS Correlation ............................................................................................................. 386
5.4.21.4 Map Filter ..................................................................................................................... 387
5.4.21.5 NandB Analysis ............................................................................................................. 388
5.4.22 Processing - Adjust ....................................................................................................... 391
5.4.22.1 Burn in Brightness and Contrast .................................................................................... 391
5.4.22.2 Interpolate Brightness and Contrast .............................................................................. 392
5.4.22.3 Channel Shift ............................................................................................................... 393
5.4.22.4 Shading Correction ....................................................................................................... 394
5.5 Maintain Tab .............................................................................................................. 395
5.5.1 Maintain - Adjust Pinhole and Collimators .................................................................... 396
5.5.1.1 Pinholes Section Panel .................................................................................................. 397
5.5.1.2 Collimators Section Panel .............................................................................................. 398
5.5.1.3 How to Adjust the Pinhole and Collimator ..................................................................... 399
5.5.2 Maintain - Objectives ................................................................................................... 402
5.5.2.1 Objective ...................................................................................................................... 402
5.5.2.2 Select Objective ............................................................................................................ 403
5.5.2.3 Add Objective ............................................................................................................... 404
5.5.2.4 Remove Objective ......................................................................................................... 405
5.5.2.5 Show/Edit Objective ...................................................................................................... 405
5.5.2.6 Focus Speed ................................................................................................................. 406
5.5.2.7 Parfocal Correction ....................................................................................................... 406
5.5.3 Maintain - Filters .......................................................................................................... 407
5.5.4 Maintain - Airyscan ...................................................................................................... 408
5.5.5 Options - System Options ............................................................................................. 409
5.5.5.1 System Options - Load Configurations .......................................................................... 410
5.5.5.2 System Options - Reuse ................................................................................................ 410
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Contents
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ZEISS
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