Zeiss LSM 880 Operating Manual page 396

ZEISS
Advanced Options
Display Negative Values shifts the offset such that also negative values are displayed as a greylevel
above zero.
Subset Length (Frames)allows to define subsets of time series and have a value processed for each
subset. This might be helpful for the analysis of time series with a high number of frames.
• The resulting output image using these settings is shown in the Preview tool and the Preview View
tab in the image container.
• Click Apply to process the image series. For each input channel, the analysis produces three output
channels: The original intensity image, I, the Number image, N, and the Brightness image, B or, if
selected, the Number image n and the brightness image e.
• Use the Coloc View Tab to display the result as a scatter plot where either the Number or Brightness
image is plotted against the intensity image.
• The table shows the mean number or brightness value along with the mean intensity value of the total
image or a selected area. This allows for example to distinguish between regions where molecules are
present as monomers or dimers.
See also the following literature for further information on N&B analysis:
Mapping the Number of Molecules and Brightness in the Laser Scanning Microscope, Michelle A. Digman
et al, Biophysical Journal Volume 94 March 2008 2320–2332
Determination of Particle Number and Brightness Using a Laser Scanning Confocal Microscope Operating
in the Analog Mode, ROOSHIN B. DALAL et al, MICROSCOPY RESEARCH AND TECHNIQUE 71:69–81
(2008)
390
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
000000-2071-464
LSM 880
10/2014 V_01