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LSM 510
Zeiss LSM 510 Manuals
Manuals and User Guides for Zeiss LSM 510. We have
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Zeiss LSM 510 manuals available for free PDF download: Manual, Quick Start Manual
Zeiss LSM 510 Manual (606 pages)
Brand:
Zeiss
| Category:
Microscope
| Size: 33.4 MB
Table of Contents
Chapter 1 Notes on Device Safety
5
Table of Contents
5
General
6
Warning and Information Labels
7
Regulations
15
Notes on Setting up the Microscope System
16
Power Requirements
18
Notes on Handling the Laser Components
19
Environmental Requirements
21
Physical Dimensions
21
Notes on Handling the Computer and Data Media
22
Notes on Care, Maintenance and Service
23
Mounting and Dismounting Lamps, TPMT and Switching Mirror
24
User Interface
24
Mounting and Dismounting the Scan Head
25
Chapter 2 Lsm 510 and Lsm 510 Meta - Setup Requirements
31
LSM on Large System Table (One Microscope, Upright or Inverted): 2 M × 3,50 M
32
Space Requirements
32
LSM on Small System Table (One Microscope, Upright or Inverted): 2 M X 3,5 M
33
LSM on Passive Anti Vibration Table (One Microscope, Upright or Inverted): 2,2 M X 4,2 M
34
LSM with Ar UV Laser
35
LSM 510 or LSM 510 META Equipped with LSM Duoscan UV
37
LSM Prepared for Two Photon Laser (NLO)
38
Power Requirements
40
Physical Dimensions
44
Dimension of Shipment Crates
45
Environmental Requirements
45
Microscopes
46
Vibrations
46
Scanning Module LSM 510 and LSM 510 META
47
Scanning Module LSM Duoscan
47
Laser Module LIVE for LSM Duoscan
48
Laser Module RGB (458, 477, 488, 514, 543 or 561,594, 633 Nm)
48
Laser Module UV (351, 364 Nm)
48
Laser Module V (405 Nm)
48
System Overview LSM 510 META
49
System Overview LSM 510 META Duoscan
51
Chapter 3 Introduction to Laser Scanning Microscopy
53
Principle of Laser Scanning Microscopy
54
Three-Dimensional Presentations of LSM Image Stacks
55
Optical Diagram of the LSM 510 and LSM 510 META (Schematic)
56
Optical and Mechanical Aspects
57
Performance Features of the LSM 510 and LSM 510 META
57
Microscope Equipment of the LSM 510 and LSM 510 META System
58
Computer Hardware and Software
60
Chapter 4 Operation in Expert Mode
63
Operation in Expert Mode
71
Purpose
71
Software
71
Windows and Window Elements
72
Backup
73
Convention for the Text in this Manual
73
Software Operation
73
Switching on the System
74
Switching on the Enterprise UV Laser
75
Starting the LSM 5 Program
76
Main Menu
78
File Menu
80
Create New Image Database
81
Image Database Window
82
Open Existing Image Database
82
Form Display Mode
84
Gallery Display Mode
86
Load an Image into the Image Display Window
87
Copy / Paste Image(S) to the Clipboard
88
Load Image with Reduced Resolution (Subset)
88
Update Image Database Display (Refresh)
88
Display Images with Certain Features (Filter)
89
Delete Function
91
On Filter Function
91
Save an Image to the Image Database
92
Import of Images
95
Export of Images
96
Multi Print
97
Overlay Toolbar
97
Exit the Expert Mode
99
Printing Images
99
Acquire Menu
100
Function Description
101
Laser Control
101
Opening / Closing the Laser Control Window
101
Settings
102
Microscope Control
103
Open the Microscope Control Window
103
Microscope Control Window for Axio Imager.z1
104
Microscope Control Window for Axiovert 200 M
109
Microscope Control for Axioskop 2 FS
113
Working in LSM Mode
113
Configuration Control
114
Open / Close the Configuration Control Window
115
Spectra Button
115
Config Button
116
Laserline Button
116
Settings for Single Track in the Channel Mode
118
Settings for Multi Track in the Channel Mode
123
Ratio Settings Panel
127
Settings in the Lambda Mode
128
Settings in the Online Fingerprinting Mode
130
Non Descanned Panel
131
Camera Detection Panel
132
Scan Control
133
Open / Close the Scan Control Window
134
Frame
136
Line
154
Spot
159
Camera Control
160
Edit ROI (Region of Interest)
162
Function Description
162
Open / Close the Edit ROI Window
162
Open / Close the Time Series Control Window
166
Time Series
166
Start Series Panel
167
Stop Series Panel
169
Cycle Delay / Time Interval Panels
170
Marker Panel
172
Time Series of a Frame
174
Time Series of a Frame over Z Stack
176
Time Series of a Frame over a Z Stack over Lambda
177
Time Series with Mean ROI
178
Edit Bleach
180
Open / Close the Edit Bleach Window
180
Settings Panel
181
Bleach Parameter Panel
182
Excitation of Bleach Track Panel
183
Stage
184
Start / End a Bleaching Procedure
184
Focus Position Panel
185
Open / Close the Stage and Focus Control Window
185
Stage Position Panel
186
Tile Scan Panel
188
VIS, TV and LSM Buttons
190
Add
191
Open / Close the Add Window
191
Process Menu
191
Destination Panel
192
Source Panel
192
Add Panel
193
Preview Panel
193
Open / Close the Subtract Window
194
Performance of the Subtract Function
194
Subtract
194
Multiply
195
Open / Close the Multiply Window
195
Performance of the Multiply Function
195
Open / Close the Ratio Window
196
Performance of the Ratio Function
196
Ratio
196
Copy (Channel)
197
Formula Panel
197
Open / Close the Copy Window
197
Performance of the Copy Function
197
Duplication (Image)
198
Filter
198
Image Panel
198
Open / Close the Filter Window
198
Filter List and Edit Panel
199
Contrast
202
Preview Panel
202
Image Panel
203
Interpolate
203
Open / Close the Interpolate Brightness and Contrast Window
203
Interpolation Panel
204
Channel Shift
205
Open / Close the Channel Shift Window
205
Preview Panel
205
Image Panel
206
Shift Panel
206
Preview Panel
207
Definition of Channels Panel
208
Open / Close the Unmix Window
208
Source Panel
208
Unmix
208
Function Description
211
Ion Concentration
211
Open / Close the Ion Concentration Window
211
Single Wavelength Dyes - Offline Calibration
212
Ratiometric Dyes
213
Ratiometric Dyes - Online Ratio
213
Ratiometric Dyes - Calibration
214
Kinetic
215
Open / Close the Kinetic Analysis Window
215
Options for Calibration Image Selection (Equation- or Titration Calibration)
215
Ratiometric Dyes - Equation Calibration (Grynkiewicz)
215
Function Description
216
Example: FRAP Performed in a Nucleus Expressing GFP Labeled Proteins
217
Copy Full Resolution
220
Copy Window Contents
220
Image Scaling
220
Paste Bitmap
220
Depthcod (Color Coded Depth Map)
221
Open / Close the Depth Coding Window
221
View Menu
221
Depth Coding Panel
222
Source Panel
222
Open / Close the Projection Window
224
Preview Panel
224
Projection
224
Source Panel
224
Projection Panel
225
Preview Panel
226
Open / Close the Stereo Images Window
228
Source Panel
228
Stereo
228
Stereo Images Panel
229
Preview Panel
231
Deconvolution (DCV)
232
Open / Close the 3D Deconvolution Window
233
Deconvolution Panel
234
Source Panel
234
Macro Language
236
Macro Menu
236
Edit Macro Function
237
Macro Control
237
Open / Close the Macro Control Window
237
Assign Macro to Button Function
240
Editing and Debugging of Macros
241
Overview of Available Macros
242
Sample Macros
244
Scanfieldtransform Macro
244
Distance Macro - Distance.lvb
246
Bleach Macro - Bleach.lvb
247
Profile Macro - Profile.lvb
247
Excitation Fingerprinting
248
Concatenate Images
252
Multiple Time Series Macro
254
Stitchart Plus Macro
255
Open / Close the Visual Macro Editor Window
265
Visual Macro Editor
265
Function Description
266
Editing a Macro
271
Export RM Function
274
Options Menu
274
Load RM Function
275
Dye DB Function
277
Settings Function
278
Spectra DB Function
278
Autosave
279
Open / Close the Settings for User
279
Database General
280
Database Gallery Viewer
281
Database Table Viewer
281
Image Status Display
282
Import / Export
282
Scan Information
282
Print Status Display
283
Recording / Reuse
283
Time Series
283
Scan Mean of Rois
284
Hardware
285
Program Start
285
Temporary Files
285
Image Display
286
Save
287
Electrical Calibration
288
Maintain Menu
288
Scanner
288
Optical Calibration with Speed 11-13 (Bidirectional)
289
Important Notes and Hints
294
Scanner Calibration in LSM 5 Software
295
Objective
296
Objective Change
296
Focus Speed Change
298
Parfocality Correction
298
Open / Close the Pinhole & Collimator Control Window
299
Pinhole Adjustment
299
Pinhole Panel
300
Collimator Panel
301
Pinhole and Collimator Adjustment
302
Set Find
304
Spline
304
Camera
305
Hwadmin
305
Test Grid
305
Close All Image Display Windows
306
Full Screen
306
Window Menu
306
Scan and System Information
307
About
308
FRAP Guide
308
Help
308
Help Menu
308
Display and Analysis of Images
309
Structure of the Image Display Window
309
Display Modes
310
Select - Chan
311
Assigning Another Color to a Channel
312
Defining a New Color
312
Switching a Channel of a Multi Channel Image off or on
312
Switching to Monochrome Image Display
312
Select - Zoom
313
Select - Slice
314
Functional Description
315
Select - Overlay
315
Buttons in the Overlay Toolbar
316
Select - Contr
319
Polyline
320
Ramp
320
Gamma
321
Spline
321
Select - Palette
322
Editing and Storing a Palette
323
Delete a Palette
324
Import a Palette
324
Select - Anim
324
Select - Reuse
326
Select - Crop
327
Select - Copy
328
Select - Save
328
Display - Xy
329
Select - Save as
329
Display - Split Xy
330
Display - Ortho
331
4.13.17.1 Ortho - Select Function
332
4.13.17.2 Ortho - Distance Function
333
4.13.17.3 Ortho - 2D Deconvolution Function
334
Display - Cut
335
Display - Gallery
336
4.13.20.1 Display - Histo - Overview
338
Display - Histo
338
4.13.20.2 Area Function
340
4.13.20.3 Colocalisation Function
342
4.13.21.1 Function
346
Display - Profile
346
4.13.21.2 Function Elements
348
4.13.22.1 Direct Setting in the Image
350
4.13.22.2 Setting Via Scrollbars
350
Display - 2.5 D
350
Display - 3D (Image Visart)
352
4.13.23.1 Shadow Projection
353
Transparency Projection
353
4.13.23.3 Surface Rendering
357
Appearance (Settings)
358
4.13.23.5 Series
359
Display - Topography for LSM
361
4.13.24.1 Channel Selection
362
4.13.24.2 Generate Topography
362
4.13.24.3 Topography Thresholds
363
4.13.24.4 Processing by Filtering
364
4.13.24.5 Display Modes
367
Context Menu of the 3D Display Mode
370
4.13.24.7 Measurement Functions
377
4.13.24.8 3D Measurement Functions
387
4.13.24.10 Topo Reuse
391
4.13.24.9 Export Data
391
Display - Prev
392
4.13.25.1 Context Menu for Scan Information Text
393
4.13.25.2 Context Menu for Topography Images
393
Arranging and Printing the Print Preview
393
Display - Info
394
4.13.27.1 Display - Mean
395
Additional Display Mode in Time Series
395
4.13.28.1 Display - Coded
399
4.13.28.2 Display - Max
399
Additional Display Modes in Lambda Mode
399
4.13.28.3 Display - Mean
400
Functionality for Emission Fingerprinting
403
4.13.28.5 Automatic Component Extraction
405
Image Optimization
407
Requirements
407
Single Channel
407
Pinhole / Detector Gain / Ampl. Offset / Ampl. Gain
411
Scan Speed, Scan Average and Pixel Depth
414
Multiple-Channel
415
Requirements
415
Image Optimization
416
Extension of the LSM 510 or LSM 510 META with LSM Duoscan
417
Exiting the LSM Program
418
Shut-Down Procedure
418
Shut down the WINDOWS Operating System
419
Turning Power off
419
Software
420
Software and Hardware Options
420
Hardware
422
Courses on "How to Operate the System in an Optimized Way
423
Chapter 5 Vba Programming for Lsm
425
General Syntax
426
VBA Programming
426
Vba Programming for Lsm
426
Working with Files
426
Access to Windows API Function and External Dlls
428
Object Structure
432
Programming for LSM
432
Differences in Access to LSM Hardware with Lsm5Hardware Object / Dsrecording Object
434
Access to Hidden Interface
437
Access to Scanned Pictures
438
Backup Recording
440
Events
441
Chapter 6 Routine Mode and Tools
443
General
444
Routine Mode
444
Routine Mode and Tools
444
Starting the Routine Mode
444
Application of Examination Methods in the Routine Mode
446
Acquisition of a Frame
447
Acquisition of a Z Stack
451
Acquisition of a Time Series
456
Change Filters
461
Tools
461
Stand Select
464
LSM Image Browser
465
LSM Image Examiner
466
Table of Contents
467
For LSM
468
Overview and Explanations
468
The Image Sequence
468
Introduction
469
Memory Usage
469
The Image Properties
469
User Interface
469
Display Window
470
Main Window
470
Functions
477
Functions in the File Menu
477
Functions in the Edit Menu
480
Functions in the Process Menu
483
Functions in the View Menu
509
Functions in the Measurement Menu
516
Chapter 8 Annex
527
Annex
528
Beam Path Configuration Guide for Fluorescence Microscopy with the LSM 5
528
Emission Filters (EF)
528
Main Dichroic Beam Splitter (HFT)
528
Optical Elements in the Configuration Control Window
528
Secondary Dichroic Beam Splitter (NFT)
528
Miscellaneous
529
Setup of Single Tracks Using Single Detectors
529
Multi Track Configuration
531
Beam Path Configuration for Multi Photon Excitation
532
Recommendations for Excitation Laser Lines and Emission Filters of Dyes
533
Configurations Overview
535
Filter Change in the Detection Beam Path of Channels 1 and 2
539
Piezo Objective Focussing Device
540
Specifications of Trigger-Interface LSM 510
541
Monitor Diode
544
NLO Non Linear Optics Laser for LSM 510 NLO and LSM 510 META NLO
551
8.8.1 Laser Control Window
552
8.8.2 Configuration Control Window
553
Non Descanned Detection (NDD)
554
Pinhole and Collimator Settings
554
Axiocam High Resolution Digital Cameras
556
High Resolution Microscopy Camera Axiocam Hrc Rev.2/3.3V
556
High Resolution Microscopy Camera Axiocam Hrm Rev.2/3.3V
556
Microscope Camera Port Adapters for the Axiocam
557
List of Key Words
558
Table of Contents
565
Multiphoton Laser Scanning Microscopy
567
Preface
567
Introduction to Multiphoton Laser Scanning Microscopy
568
Multiphoton Excitation - How Does It Work
568
Increased Signal-To-Noise, Enhanced Vitality, and Deep Optical Sectioning in MPLSM
570
Drawbacks of Using NIR Light for Microscopy
571
Achieving Efficient Multiphoton Excitation Using Ultrafast Lasers
572
Optimizing the Peak Intensity Without Frying the Samples
573
Coupling an Ultrafast Laser to the LSM 510 NLO
575
Using the LSM 510 NLO Direct Coupled System: Practical Considerations for
575
Alignment of the Ultrafast Laser into the LSM 510 NLO Scan Head
576
Quick Alignment Protocol
576
Advanced Alignment Protocol
578
Tips on Maintaining Alignment with the Scan Head
579
Choosing Fluorescent Probes for MPLSM
582
Samples Your Mother Should Have Warned You about
584
No Image Being Produced
587
Troubleshooting Checklist
587
Poor Image Quality
588
References
589
Starting the System
593
Setting the Microscope
596
Configuring the Beam Path and Lasers
598
Scanning an Image
601
Storing an Image
605
Switching off the System
605
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Zeiss LSM 510 Quick Start Manual (26 pages)
Brand:
Zeiss
| Category:
Medical Equipment
| Size: 2.37 MB
Table of Contents
Table of Contents
2
Start Hardware
3
Start Software
4
Start Lasers
5
Find the Specimen
6
(Axioplan 2)
6
Find the Specimen
7
(Axiovert 200M)
7
Confocal Filter Set Configuration
8
Acquire Preliminary Confocal Image
9
Optimizing the Settings
10
Getting the Display Ready
10
Scan Control: Channels Window
12
Acquiring Your Final Image
15
Saving Your Final Image
17
Acquiring a Z-Series
18
Advanced Options
20
Single-Track - Simultaneous Acquisition
20
Getting the Display Ready
20
Optimise Settings
20
Z-Attenuation Compensation
21
Transmitted Light Image
22
Frap
23
Shutting down the System
25
Turn off Lasers
25
Remove Specimen and Clean Microscope
25
Exit the Software
25
Power down the System
25
Opening Your Images Offline
26
Zeiss Image Browser
26
Imagej
26
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