Action Buttons - Zeiss LSM 880 Operating Manual

LSM 880
5.2.3

Action Buttons

Action buttons provide control for how the image is acquired (Fig. 76).
Fig. 76 Action buttons
They have the following functions, regardless of the imaging mode:
AF Find Focus triggers a frame z-Scan. The system selects the z-position associated with the frame of
highest intensity or contrast. AF Find Focus can be disrupted by pressing the Stop button, to which the
Snap button changes upon activation (Fig. 77).
Fig. 77 Action buttons – AF Find Focus button active
Set Exposure optimizes the gain and offset settings (PMT only, for cameras only the exposure time will
be adjusted) of all active channels and tracks dependent on the given laser power. Set Exposure can be
disrupted by pressing the Stop button, to which the Set Exposure button changes upon activation
(Fig. 78).
Fig. 78 Action buttons – Set exposure button active
Live uses the maximum speed for a given image frame (and zoom setting) for all active channels. It wil
set the resolution to 248 x 248 for the Andor SIM camera, 256 x 256 for the Andor PALM camera and
512 by 512 for single point detectors (PMT, GaAsP). To disrupt Live acquisition, the Stop button has to
be pressed into which the Live button changes after its activation (Fig. 79).
Fig. 79 Action buttons – Live button active
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
000000-2071-464
ZEISS
73