Zeiss LSM 880 Operating Manual page 13

LSM 880
6.11 Colocalization View ................................................................................................... 472
6.11.1 How a Scatter Diagram is Generated ............................................................................. 474
6.11.2 Quantitative Colocalization Parameters Shown in the Data Table .................................... 475
6.11.3 Colocalization Coefficients ............................................................................................ 475
6.11.4 Overlap Coefficient, Overlap Coefficient after Manders .................................................. 476
6.12 Profile View ................................................................................................................ 476
6.13 Shuttle and Find View ............................................................................................... 479
6.14 Mean of ROI: Additional View Type for Time Series................................................ 482
6.15 Kinetic / FRAP View: Additional View Type for Time Series .................................... 486
6.16 Lambda Coded: Additional View Types for Lambda Mode ..................................... 492
6.17 Unmixing View .......................................................................................................... 494
6.17.1 Automatic Component Extraction ................................................................................. 499
6.18 FRET View ................................................................................................................... 501
6.18.1 Tools in the FRET View Options Control Block for Acceptor Photobleaching .................... 503
6.18.2 Tools in the FRET View Options Control Block for Sensitized Emission ............................. 505
6.19 Raster Scanning Image Correlation Spectroscopy (RICS) ......................................... 508
6.19.1 Access to RICS .............................................................................................................. 509
6.19.2 Control of View Functionalities ...................................................................................... 509
6.19.3 Correlation Functionalities ............................................................................................. 510
6.19.4 Display Functionalities ................................................................................................... 513
6.19.5 Fitting Functionalities .................................................................................................... 520
6.19.6 Model Functionalities .................................................................................................... 523
6.20 Polarization Imaging ................................................................................................. 527
6.20.1 Imaging Setup for Polarization Imaging ......................................................................... 528
6.20.2 Determination of G-factor ............................................................................................. 529
6.20.3 Obtaining an Anisotropy Image ..................................................................................... 530
6.21 Information View ....................................................................................................... 532
7 RIGHT TOOL AREA, DATA MANAGEMENT AND STORAGE ........................... 534
7.1 General ....................................................................................................................... 534
7.2 ZEN File Browser ........................................................................................................ 535
7.2.1 Gallery View of the ZEN File Browser ............................................................................. 538
7.2.2 Form View of the ZEN File Browser ................................................................................ 540
7.2.3 Table View of the ZEN File Browser ............................................................................... 541
7.3 Images and Documents Panel ................................................................................... 542
7.4 Opening of Files via the "Open" Command in the File Menu ................................. 545
7.5 Save ............................................................................................................................ 545
7.6 Export of Images ........................................................................................................ 546
8 INDEX ............................................................................................................... 547
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Contents
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ZEISS
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