Multidimensional Acquisition - Z-Stack - Zeiss LSM 880 Operating Manual

LSM 880
In this case the scanner movement will be restricted to the bleach region zooming in onto this region.
This may result in a less accurate positioning of the region as the definition of the region has been made
in a different zoom in the image.
The bleach process will be faster than without this option. The gain in speed is dependent on the speed
that is used for bleaching. If a high speed has already been chosen for bleaching the gain in speed will be
minimal.
This function will only be applied for the LSM imaging mode. For widefield modes (WF, SIM, TIRF) it
will be ignored
The numbers of the different regions will be shown in the image if the check box next to Show
numbers is checked.
Regions can be saved by pressing the Save... button using Windows Explorer.
This process stores all regions currently displayed in the Regions tool in one file. Saved images can be
loaded later on by pressing the Load... button using the Windows Explorer.
5.2.15 Multidimensional Acquisition –
Z-Stack
The Z-Stack tool tab is only available, if the Z-
Stack checkbox is ticked in the Multidimensional
acquisition field. If the tool bar is expanded, the
Z-Stack tool becomes available (Fig. 192). When
Start Experiment is pressed the system will
perform a Z-Stack.
With the Z-Stack function a series of XY-images in
different focus positions can be acquired resulting
in a Z-Stack. In this way a 3 dimensional data set
from the specimen is obtained.
The way in which the Z-Stack is acquired can be
selected by pressing either the First/Last or
Center mode buttons. The modes differ in the way
the first and last images of the stack are selected.
The Center mode is only available in the Show all
mode.
In case a Z-Piezo stage insert focusing device is
attached to the system, the Piezo device can be
chosen for the acquisition of the stack.
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Fig. 192 Z-Stack tool First/Last mode with
Z-Stack Navigation displayed for
the LSM image mode
ZEISS
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