Imaging Setup For Polarization Imaging - Zeiss LSM 880 Operating Manual

ZEISS Center Screen Area / Image Containers - Display and Image Analysis
Fig. 745 Imaging Setup with Notch filter
control button
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CHAPTER 1 - SYSTEM OPERATION
6.20.1
In case emission polarizers (analyzers) are available,
you have a Notch filter cascade selection button
available situated on top of the MBS button (see
Fig. 745).
Set up a multitrack configuration using exactly the
same settings with the exceptions of the analyzers.
To this end press the Notch filter cascade button.
This opens up the Polarizer selection box. Check
the polarizer which you want to use with track 1.
The options are P (for the parallel polarized light
transmission) and S (for vertical polarized light
transmission) (see Fig. 746).
If you want to ratio image, then it is best for
convenience to choose P for track 1. Check the
other polarizer for track 2.
For ratio imaging this would be S. For ratio
imaging check the R1 channel box and assign
Source 1 to the selected channel-T1 and Source 2
to the selected channel-T2. You can use the single
PMTs Ch1 and Ch2 or the spectral detector ChS.
Fig. 746
000000-2071-464
Imaging Setup for
Imaging
Imaging Setup with Notch filter
cascade option
LSM 880
Polarization
10/2014 V_01