Zeiss LSM 880 Operating Manual page 270

ZEISS
(1) Positioning in solution
• Align a carrier in the Carrier Position tool. Focus into the solution.
• Go to the Measure tool and the Acquisition panel. Choose Current Position or Sample Carrier
and select the well(s) for the measurement.
(2) Positioning in a cell
Before positioning you have to select appropriate cells and image those. An overview of fluorescent
expressing cells is best achieved by using the binoculars in transmitted and epi-fluorescent mode. Once
cells with adequate expression levels have been identified they can be imaged with the LSM.
Alternatively, suitable cells can be searched by using low zoom values in the LSM.
Fig. 352 Microscope Control tool
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Visual inspection of the cells via binoculars
• In the main toolbar, activate the Locate tab.
• Open the Microscope Control tool (Fig. 352).
The Microscope Control tool permits motorized
functions including objective and reflector change
settings as well as the illumination mode like
transmitted or reflected light of the connected
microscope to be controlled via the software.
These microscope functions can also be
operated directly on the microscope stand
via assigned buttons or through the touch
screen, if available. In that case, any changes
are recorded by the software and displayed
in the relevant windows / panels.
For side and base port systems, the reflector
has to be on a none position.
LSM 880
10/2014 V_01