Zeiss LSM 880 Operating Manual page 666

4.5
Optical slice
(NA = 1.3; n = 1.52;
4.0
λ  = 496 nm)
3.5
3.0
2.5
2.0
1.5
1.0
0.5
1000
Depth resolution
920
(PH = 0; n = 1.52;
λ  = 496 nm)
840
760
680
600
520
440
360
280
200
600
Depth resolution
(PH = 0; NA = 1.3; n = 1.52) 560
520
480
440
400
360
320
280
240
200
1600
Depth resolution
(PH = 0; NA = 0.8; 1520
λ  = 496 nm)
1440
1360
1280
1200
1120
1040
960
880
800
16
0 0.5 1.0 1.5 2.0 2.5
Pinhole diameter [AU]
1 1.1 1.2
Numerical aperture
488 504 520 536 552
Wavelength [nm]
1.33 1.36 1.38 1.41 1.44
Refractive index of immersion liquid
3.0 3.5 4.0 4.5 5.0
1.3 1.4
568 584 600
1.47 1.49 1.52
Fig. 11
a) Variation of
pinhole diameter
b) Variation of
numerical aperture
c) Variation of
wavelength (λ  )
d) Variation of
refractive index
fluorescent plane
fluorescent point
reflecting plane (mirror)