Multidimensional Acquisition - Tile Scan - Zeiss LSM 880 Operating Manual

ZEISS
Fig. 204 Tile Scan tool – Centered grid active
Fig. 205 Tile Scan tool – Overlap defined
Fig. 206 Tile Scan tool – Marked positions
If the system is equipped with the software option TILES, the Overlap input box is available (Fig. 205).
Define the overlap of the tiles as the % of the tile size. The extent of overlap will also be displayed in the
grid display area. As soon as the Overlap is > 0 % the Online stitching checkbox becomes available.
Online stitching cannot be activated if the HDR imaging is selected in the Acquisition Mode tool.
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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5.2.17 Multidimensional Acquisition – Tile
Scan
When the Tile Scan tool tab is expanded the Tile
Scan tool becomes available (Fig. 204).
This function permits a frame to be created as an
overview image of the specimen. The size of the
final tiled image depends on the settings for the
image format set in the Acquisition Mode tool
and the number of tiles.
The application of the Tile Scan function requires
an objective with a minimum magnification factor
of 2.5x.
The number of Tiles can be defined in the Tiles
Horizontal and Vertical Input boxes. A maximum
of 100 tiles can be set. The size of the final Tile
Scan is displayed in Pixels and as a Size in µm.
According to image format the number of Pixels
changes. According to the objective used for
imaging the Size of the Tile Scan changes.
The Tile Scan grid is displayed in the grid display
area. It shows the number and location of the
single tiles and also indicates any overlap.
The Tile scan is created around the absolute
starting position of the stage. In case the stage
does not move exactly horizontal to the scan field
a Rotation value can be set in the Rotation input
box to correct for this (available only in Show all
mode). This can either be calibrated beforehand
using a grid slide or any other specimen which
displays clearly visible structures in horizontal
direction.
Acquire smaller tile scans to see the effect of a
specific value for Rotation until you find the right
one. This value should be taken for the Rotation.
Alternatively, use the Tile Scan Rotation Macro
which provides an automatic Rotation calibration
function. Take over this value to the main software
before acquiring the tile scan.
When Bi-directional is active (available only in
Show all mode) the image acquisition is
performed also on the horizontal backwards
movement of the stage.
LSM 880
10/2014 V_01