Zeiss LSM 880 Operating Manual page 104

ZEISS
Fig. 117 Imaging Setup tool: WF mode
98
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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WF Mode (Widefield Camera Mode)
The WF mode (widefield camera mode) displays
the current hardware settings for an imaging track
using the camera as detection channel. The display
changes in accordance with the selection of a track
in the Imaging Setup tool.
The WF mode provides access to the conventional
illumination sources, the halogen lamp and HBO
(X-Cite 120 / HXP). It also provides access to the
condenser lens aperture and filters and the tube
lens.
The use of this function permits the use of a Zeiss
AxioCam camera (various models, see ANNEX for
description) as an alternative external detector.
The camera can be used with the transmission
illumination source halogen lamp, which allows
acquiring a standard transmission image or,
depending on the optics of the microscope, a
differential interference contrast image.
When the HBO (or X-Cite 120 / HXP) is used as an
illumination source a standard fluorescence image
can be acquired. In this case a specific fluorescence
filter cube has to be chosen in the reflector turret
filter wheel.
A corresponding tube lens (1x or 0.63x) should be
used.
LSM 880
10/2014 V_01