Zeiss LSM 880 Operating Manual page 238

ZEISS
Fig. 305 Beam path configuration for
finding glass reflection
232
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Proceed as follows:
• Make sure that the front lens of the water
immersion objective is wetted by a drop of
water. Use fluorescence-free double-distilled
water, since otherwise the immersion water will
cause background fluorescence and deteriorate
the correlation signal.
• Choose a chamber by clicking on one well in
the X, Y (Stage) panel of the Sample Carrier
tool.
• Set up a beam path in the LSM Imaging Setup
tool (Fig. 305).
Convenient settings are:
− 488 nm line at 50 % tube current and 5 %
AOTF
− MBS T80/R20 major dichroic beam splitter
for visible light
− Plate major dichroic beam splitter for
invisible light
− None for side port systems, Rear for rear port
systems at the reflector position
− Reflection check box activated
− Appropriate spectral band in the detector
channel to detect the reflected excitation
light, for example 420 – 650 nm for 488 nm
light
LSM 880
10/2014 V_01