Zeiss LSM 880 Operating Manual page 114

ZEISS
• Select the Scan Speed from the 19 preset steps via slider or input box. Recommended: 8 for the first
scan. A click on the Max button sets the maximum speed for the current zoom.
In basic mode (Show all inactive), the LSM switches automatically to bi-directional scanning if high
−
scan speeds are selected (see also description of bi-directional scanning below).
The Scan Speed determines the Pixel Dwell time. In the case of different image formats, the
−
Pixel Dwell time is constant for the same Scan Speed, but the Scan Time is different.
Pixel Dwell time of the laser beam on the pixel
−
The Scan Time duration of the acquisition for the entire frame
−
Speed:
Zoom:
Mode:
* Values refer to image format 512 x 512 pixels, 8 bit data depth and a tube lens of 150 mm (LSM on the side
port of Axio Observer); other values apply for higher formats and data depth.
• Select 8 Bit, 12 Bit or 16 Bit Data Depth, i.e. 256, 4096 or 65536 gray values.
• Select the Unidirectional or Bi-directional Scan Direction.
Unidirectional: The laser scans in one direction only, then moves back with beam
−
blanked and scans the next line.
Bi-directional: The laser also scans when moving backwards, i.e. the Scan Time is
−
halved. The online scanner calibration (OSC) will correct for nonlinear distortions of the lines in
bidirectional mode. This function (see also checkbox in Maintain Tab -> Options – System
Options-> Hardware) eliminates most of the deviations of the scanmirrors at the very fast speeds.
−
image) resulting from bi-directional scanning must be corrected. For automatic scan correction,
click on the Auto button. If Auto is off the scan Corr X and Y sliders which are indicated when
selecting bidirectional scanning. Zero° rotation requires correction in the X-direction, 90° rotation
must be corrected in the Y-direction. If the image was rotated, correction is required in both
coordinates. Correction is performed on-line in the Continuous scan mode. The size of the shift
depends on the Scan Speed.
• Select the Line or Frame mode for averaging.
• Select the desired scan average method Mean or Sum.
• Select the desired scan average from the available values 2, 4, 8 and 16 in the Number selection drop
down menu or Continuous (only for Frame average mode).
The greater the number of averages selected for Mean average Method, the better the image
quality will be; the scanning time will be prolonged accordingly.
Averaging can be performed in different ways, depending on whether the Mean or Sum method has
been activated.
If you are using the Mean method, the image information is generated by adding up all scans pixel by
pixel and then calculating the mean value.
108
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
1 ... 11 12
≤ 1.8
0.6 - 1.4
lbda./ lbda./
34 ch. 32 ch.
The pixel shift between forward and backward movement (double
000000-2071-464
13 14
≤ 2.9 ≤ 3.3
10-ch 10-ch
15 16
≤ 4.3 ≤ 5.4
6-ch 6-ch
10/2014 V_01
LSM 880
19
≤ 13.2
4-ch