Zeiss LSM 880 Operating Manual page 323

LSM 880
Ratiometric Dyes - Calibration (Fig. 419)
• Subtract background/autofluorescence images from raw images to obtain
R [=F -F
korr 1 1Background
when calibration reference is not obtained with the experimental sample (in situ).
• Calculate ratio R.
• Perform equation- or titration calibration (compare R with a calibration curve → titration calibration or
put R values in calibration formula).
Fig. 419
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
)/(F -F )]
2 2Background
Ion concentration panel
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