Single Wavelength Dyes - Offline Calibration - Zeiss LSM 880 Operating Manual

LSM 880
5.4.9.1 Single Wavelength Dyes – Offline Calibration
• Subtract background/ autofluorescence image from raw images to obtain better raw data to start with
(Fig. 417).
• Perform equation or titration calibration (compare F with a calibration curve → titration calibration or
put F values in calibration formula).
Fig. 417
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
Ion concentration panel
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ZEISS
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