Options - Fcs Options; Fcs Options - Data - Zeiss LSM 880 Operating Manual

LSM 880
5.5.6 Options – FCS Options
Open the FCS Options tool (Fig. 582). The tool has three tabs:
−
Data,
−
Measure,
−
Auto Save.
5.5.6.1 FCS Options – Data
Saving Raw Data During Measurement
If the Save raw data during measurement
check box is activated (Fig. 582), raw data will be
saved temporarily in a specified directory on disc
(Fig. 583). Each single measurement will be saved
in a single raw data file. The name of the raw data
file is an identifier to the .fcs measurement it
belongs and the channel. This option is used when
access to the raw data is required to analyze the
data in a different way in respect to correlation
and PCH settings or within other programs.
If the check-box is de-activated, no raw data
will be saved.
Temporary raw data will be deleted from the
specified file upon closing the Image FCS
diagram. You will be prompted in the Save
raw data dialog box, if you want to save the
raw data. Press Yes to save the raw data
permanently along with the .fcs file in a
specified directory, which can be defined in
the Save dialog box. Press No to discard the
raw data. Note that raw data are saved
along with the .fcs measurement file in the
same folder. They are attached to the .fcs file
via a relative file path. If you save the same
measurement again without the option Save
Raw Data, than the raw data files will be
reassigned an absolute file path and the
connection to the .fcs file is lost.
Please note, if the Auto safe checkbox is ticked in the Auto Save submenu, temporary raw data
will be stored permanently and have to be deleted manually. Care has to be taken to avoid disc
space limitations, since raw data files can be huge.
Each single measurement is stored in one raw data file. For two channel experiments, each channel
will be stored in a separate file.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
000000-2071-464
Fig. 582 FCS Options, Data
Fig. 583 Stored temporary raw data
ZEISS
413