Pch - Zeiss LSM 880 Operating Manual

ZEISS
Fig. 289 Model panel, PCH tab
− You can choose between 1, 2 or 3 components by activating the Components 1, 2 and 3 buttons,
respectively for each activated component you can enter a brightness value in Hz in the Specific
brightness enter boxes. If you don't know the brightness, enter 1.
− You can enter Instrumental parameters values in the respective first order, second order and
third order enter boxes. These values have to be determined in independent calibration
experiments using a defined dye solution with a known brightness. The parameters correct for the
deviation of the confocal volume from a true Gaussian distribution. Normally, only a correction for
the first order parameter is necessary and recommended. Hence, in the calibration fit you will keep
the first order parameter free and the second and third parameters fixed to zero. You can enter the
determined first order number and fix it for later measurements.
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
000000-2071-464
5.3.4.5

PCH

In the PCH (Photon Counting Histogram) panel you
can determine concentrations and the molecular
brightness of molecules.
• Click on the PCH tab to open the PCH control
panel (Fig. 289).
You have the following options:
− You can enter a background value, if you
know the background in the Background
display box. Enter a zero, if you assume no
background. The background is no fit
parameter.
LSM 880
10/2014 V_01