Zeiss LSM 880 Operating Manual page 10

ZEISS
5.3.4 Setup - Fit ..................................................................................................................... 192
5.3.4.1 Defining a New Model ................................................................................................... 194
5.3.4.2 Correlation Options ....................................................................................................... 195
5.3.4.3 Model Equations ........................................................................................................... 196
5.3.4.4 Definition of a Model .................................................................................................... 213
5.3.4.5 PCH .............................................................................................................................. 222
5.3.4.6 Formula ........................................................................................................................ 223
5.3.5 Setup - Correlator ......................................................................................................... 224
5.3.6 Navigation - Stage ........................................................................................................ 228
5.3.7 Navigation - Sample Carrier........................................................................................... 229
5.3.8 Navigation - Focus ........................................................................................................ 231
5.3.9 Multidimensional Acquisition Tools - Time Series ............................................................ 236
5.3.10 Multidimensional Acquisition Tools - Positions ............................................................... 238
5.3.11 Navigation - Incubation ................................................................................................. 247
5.3.12 Multidimensional Acquisition Tools - Information on Experiment .................................... 248
5.3.13 Action Buttons for FCS .................................................................................................. 249
5.3.14 Method Tool ................................................................................................................. 250
5.3.15 Activation Tab Area ....................................................................................................... 253
5.3.16 New .............................................................................................................................. 253
5.3.17 Count Rate ................................................................................................................... 254
5.3.18 X-Y-Z Scan .................................................................................................................... 256
5.3.19 Snap ............................................................................................................................. 259
5.3.20 Start Experiment ............................................................................................................ 259
5.3.21 Taking FCS Measurements ............................................................................................. 260
5.3.21.1 Start and End of a Measurement ................................................................................... 263
5.3.21.2 Loading Stored Data Files .............................................................................................. 267
5.3.21.3 FCS Correlation Diagram ............................................................................................... 268
5.3.21.4 Image View Controls - Correlation Tab .......................................................................... 271
5.3.21.5 Image View Controls - Table Tab ................................................................................... 273
5.3.21.6 Saving Correlation Results ............................................................................................. 278
5.3.21.7 Coincidence .................................................................................................................. 280
5.3.21.8 Fit ................................................................................................................................. 281
5.3.21.9 Print .............................................................................................................................. 290
5.3.21.10 Information ................................................................................................................... 292
5.4 Processing Tab ............................................................................................................ 293
5.4.1 General Structure of the Processing Tab ......................................................................... 293
5.4.2 Processing - Airyscan Processing .................................................................................... 297
5.4.3 Processing - Maximum Intensity Projection ..................................................................... 300
5.4.4 Processing - Color-coded Projection ............................................................................... 301
5.4.5 Processing - Image Calculator ........................................................................................ 302
5.4.6 Processing - Average ..................................................................................................... 304
5.4.7 Processing - Filter .......................................................................................................... 305
5.4.8 Processing - Linear Unmixing ......................................................................................... 308
5.4.9 Processing - Ion Concentration ...................................................................................... 313
5.4.9.1 Single Wavelength Dyes - Offline Calibration ................................................................. 315
5.4.9.2 Ratiometric Dyes ........................................................................................................... 316
5.4.10 Processing - Correlation ................................................................................................ 319
5.4.11 Processing - Modify Series ............................................................................................. 320
4
CHAPTER 1 - SYSTEM OPERATION
Contents
000000-2071-464
LSM 880
10/2014 V_01