Zeiss LSM 880 Operating Manual page 531

CHAPTER 1 - SYSTEM OPERATION
LSM 880 Center Screen Area / Image Containers - Display and Image Analysis ZEISS
Translation G (ξ,ψ) describes the translational diffusion term of the model function. To open the
d
Translation settings press the Settings button (Fig. 742).
Fig. 742 Settings for the Translation term
If the Fractional intensities box is checked, the Molecular brightness input boxes will become
available. Enter the absolute or relative molecular brightness of the molecules, which have to be
determined beforehand. If unchecked, molecular brightness will not be taken into account and the
Molecular brightness input box will not be displayed.
If the Diffusion coefficient check box is ticked, the diffusion coefficient will be fitted, otherwise the
diffusion time. In case the box is checked, the 2 Photon check box will be accessible. If ticked diffusion
th th 2
time scales with 1/8 of the diffusion coefficient, if un-ticked with 1/4 . The 1/e radius of the laser beam
must be entered within the ω in µm input box, which is also displayed only in case the Diffusion
r
coefficient box by typing or using the arrows.
10/2014 V_01 000000-2071-464 525