Zeiss LSM 880 Operating Manual page 503

LSM 880 Center Screen Area / Image Containers - Display and Image Analysis
Tools in the Unmixing View Options Control Block
− Tools for display customization and drawing:
Tables can always be saved or copied to the clipboard by right-mouse clicking on the table
display!
− Tools for unmixing:
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CHAPTER 1 - SYSTEM OPERATION
Drawing tools work the same as in the Graphics View Option control
block
Check box for image display: show the image in greyscale or lambda
coded (see as well section Lambda Coded: Additional View Types
for Lambda Mode)
Dialog for diagram display:
− Spline on/off
− Normalize the graphs of the spectra to 1
− Set the line width of the spectra in the diagram
− Selection of graph style
Show Table button: The table of intensity values over Lambda is
displayed in the Image Display window.
Overview of spectral data selected, that is displayed and can be used for
unmixing. Spectra saved in the spectra database can be loaded (here:
Venus) by pressing the plus button in the upper right corner which
creates a new row. In this row the left black arrowhead opens the
spectra database. Manually defined ROIs (Region) and pixel selectey by
Automatic Component Extraction (ACE) can be added.
Select ROI for background subtraction
Automatically balances the intensity of unmixed channels to equal
levels.
A statistical analysis of the signal-related (Poisson-) noise is performed
and weighs the respective contribution to the fitting of the combination
of reference spectra to the experimental data (see also section
Processing – Linear Unmixing).
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