Zeiss LSM 880 Operating Manual page 497

LSM 880 Center Screen Area / Image Containers - Display and Image Analysis
If the analysis is done using the double exponential fit the fitted curve displays the mean of the fitted
values for the two different mobile fractions. The table shows the following additional parameters:
− The amplitude of the two curves, displayed as one (which corresponds to each part of the mobile
fractions I1 and I2).
− The fitted parameters T1 and T2 (seconds) for each mobile fraction.
− The rate constant for the exchange of molecules between the bleached region and the surrounding
area K1 and K2 (per second) for each mobile fraction.
The raw data of the experiment can be exported for further analysis using the Mean ROI display
mode and, within this dialog, the table display of the results.
Note that this modeling is also a very basic approach to your experiment. It offers a first hint on
the possible presence of two mobile fractions of the labeled protein within the cell or cell
compartment examined.
Please refer to relevant scientific literature or the website of the EAMNET (http://www.embl.de/eamnet)
for further information on how to set up and perform FRAP experiments. A schematic curve marking the
data points that are calculated performing the Kinetic Analysis is shown in Fig. 683. Please note, that the
naming of the data points is not consistent with the information on the website.
A more general introduction to FRAP experiments can be found in the "Methods" brochure
"Photomanipulation with Laser Scanning Microscopes" by Carl Zeiss Microscopy GmbH (order no. 45-
0060 e) and the references cited therein.
Fig. 683
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CHAPTER 1 - SYSTEM OPERATION
Schematic FRAP curve with marks at the relevant data points.
I0, I1 and I delta which are calculated performing the Kinetic
Analysis
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